Summary
A method is described for the maceration (dissociation) of hydra tissue into single cells. The cells have characteristic morphology such that all basic types — epithelial, gland, mucous, interstitial, nematoblast, and nerve — can be distinguished. Criteria are given for identifying each cell type by phase contrast microscopy. It is shown that maceration quantitatively recovers cells from hydra tissue.
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David, C.N. A quantitative method for maceration of hydra tissue. W. Roux' Archiv f. Entwicklungsmechanik 171, 259–268 (1973). https://doi.org/10.1007/BF00577724
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DOI: https://doi.org/10.1007/BF00577724