Modified method for the quantitative determination of certain deoxy sugars in lipopolysaccharides
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An alkaline modification of the 2-thiobarbituric (2-TBA) method for the rapid determination of 2-keto-3-deoxy-D-mannooctonic acid (KDO) and of 3,6-dideoxyhexoses (3,6-DDHs) in hydrolysates of lipopolysaccharides is described. The modification is based on the performance of the periodate-2-TBA color reaction with the subsequent decomposition of the KDO chromophore by alkaline treatment and measurement of the optical densities of the solutions before and after this treatment. It has been found that the modification described is distinguished by high sensitivity, specificity, and reproducibility of the results. The standard deviation calculated from six determinations amounted to 2.84% for KDO and 3.93% for 3,6-DDHs. The method is simple to perform and needs only simple equipment and a small amount of reagents.
KeywordsXylose Periodate Uronic Acid Galacturonic Acid Alkaline Treatment
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