Abstract
We have previously reported that (a) polyclonal antisera raised against rat Sertoli cell secretory protein S70 and S45-S35 heterodimeric protein recognize outer dense fiber polypeptides from rat sperm tail, and (b) protein S70 is antigenically related to polypeptides S45 and S35, the disulfide-linked components of the heterodimeric protein. We now report that polyclonal antisera generated against three different outer dense fiber polypeptides recognize (a) the putative antigen of the sperm tail and (b) Sertoli cell secretory protein S70 and its antigenically-related polypeptides. Immunogold electron microscopy shows that outer dense fibers of epididymal sperm crossreact with anti-S70 serum as well as with an antiserum raised against the polypeptide D complex of extracted outer dense fibers. Electron microscopy demonstrates that outer dense fibers consist of filamentous, coil-coiled units aligned side-by-side with each other. Results of this study strengthen the antigenic homology between Sertoli cell secretory proteins and outer dense fiber polypeptides of the sperm tail.
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References
Fawcett DW: The mammalian spermatozoon. Dev Biol 44:394–436, 1975
Calvin HI, Bedford JM: Formation of disulphide bonds in the nucleus and accessory structures of mammalian spermatozoa during epididymal maturation. J Reprod Fert Suppl 13:65–75, 1971
Bedford JM, Calvin HI: Changes in -S-S- linked structures of the sperm tail during epididymal maturation. with comparative observations in submammalian species. J Exp Zool 187:181–204, 1974
Calvin HI, Hwang FH-F, Wohlrab H: Localization of zinc in a dense fiberconnecting piece fraction of rat sperm tails analogous chemically to hair keratin. Biol Reprod 13:228–239, 1975
Vera JC, Brito M, Zuvic T, Burzio LO: Polypeptide composition of rat sperm outer dense fibers. A simple procedure to isolate the fibrillar complex. J Biol Chem 259:5970–5977, 1984
Irons MJ, Clermont Y: Formation of the outer dense fibers during spermiogenesis in the rat. Anat Rec 202:463–471, 1982
Shabanowitz RB, DePhilip RM, Crowell JA, Tres LL, Kierszenbaum AL: Temporal appearance and cyclic behavior of Sertoli cell-specific secretory proteins during the development of the rat seminiferous tubule. Biol Reprod 35:745–760, 1986
Kierszenbaum AL, Abdullah M, Ueda H, Tres LL: Spermatogenesis in vitro: Searching for in vivo correlates. In: VB Mahesh, DS Dhindsa, E Anderson SP Kalra (eds) Regulation of Ovarian and Testicular Function. Plenum Press, New York, 1987, pp 535–560
Abdullah M, Tres LL, Ueda H, Hu P-H, Kierszenbaum AL: Antigenic homology between rat sperm tail polypeptides and Sertoli cell secretory proteins. Mol Cell Biochem 81:165–176
Kierszenbaum AL, Ueda H, Ping L, Abdullah M, Tres LL: Antibodies to rat Sertoli cell secretory proteins recognize antigenic sites in acrosome and tail of developing spermatids and sperm. J Cell Sci 91:145–153
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685, 1970
Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354, 1979
Burnette WN: ‘Western blotting’: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrilamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203, 1981
Creighton TE: Proteins. Structures and Molecular Principles. WH Freeman, New York, 1983, pp 159–197
Olson GE, Sammons DW: Structural chemistry of outer dense fibers of rat sperm. Biol Reprod 22:319–322, 1980
Baccetti B, Pallini V, Burrini G: The accessory fibers of the sperm tail. III. High sulfur and low sulfur components in mammals and cephalopods. J Ultr Res 57:289–308, 1976
Calvin HI: Selective incorporation of selenium-75 into a polypeptide of the sperm tail. J Exp Zool 204:445–452, 1978
Calvin HI, Grosshans K, Musicant-Shikora SR, Turner SI: A developmental study of rat sperm and testis selenoproteins. J Reprod Fert 81:1–11, 1987
Kierszenbaum AL, Crowell JA, Shabanowitz RB, Smith EP, Spruill WA, Tres LL: A monoclonal antibody recognizes a form of intermediate filament protein in rat Sertoli cells that is not present in seminiferous peritubular cells. Biol Reprod 35:227–238, 1986
Nelson WJ, Traub P: Proteolysis of vimentin and desmin by the Ca2+-activated proteinase specific for these intermediate filament proteins. Mol Cell Biol 3:1146–1156, 1983
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Kierszenbaum, A.L., Abdullah, M., Ueda, H. et al. Antibodies to rat sperm tail polypeptides recognize Sertoli cell secretory proteins. Mol Cell Biochem 85, 171–179 (1989). https://doi.org/10.1007/BF00577112
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DOI: https://doi.org/10.1007/BF00577112