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Quantittive determination of amino sugars in bacterial lipopolysaccharides

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Abstract

A modification of the method of quantitatively determining amino sugars in polysaccharide preparations of bacterial origin is described which excludes the influence of the mixture of amino acids and monosaccharides on the result of the analysis. The modification is based on differences in the extraction capacity of organic solvents in relation to the various chromophores obtained in the Elson-Morgan reaction from amino sugars and from the mixture of amino acids and monosaccharides. It has been found that the modification described is distinguished by a high sensitivity, specificity, and reproducibility of the results. The standard deviation calculation for 10 determinations does not exceed 4%. The method is simple to perform and it requires uncomplicated equipment and small amounts of reagents.

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Authors
  1. T. I. Burtseva
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  2. S. A. Cherkasova
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  3. Yu. S. Ovodov
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Additional information

Pacific Ocean Institute of Bioorganic Chemistry, Far-Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 739–743, November–December, 1985.

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Burtseva, T.I., Cherkasova, S.A. & Ovodov, Y.S. Quantittive determination of amino sugars in bacterial lipopolysaccharides. Chem Nat Compd 21, 696–699 (1985). https://doi.org/10.1007/BF00576198

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  • DOI: https://doi.org/10.1007/BF00576198

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