Summary
Eye-antennal and ventral mesothoracic (midleg) disks of 7 1/2 days old, mature larvae ofD. hydei, homozygous for the lethal genelpl (lethal-polyploid), have been transplanted into 7 1/2 or 4 1/2 days old larval wildtype hosts.
The morphology oflpl disks shows a variation of damage ranging from complete absence or strongly reduced anlagen to normal sized disks. Transplanted disks are able to undergo metamorphosis and to differentiate adult structures. The inventory of structures formed varies individually and is always less complete than differentiations obtained from wildtype controls, but the topographical arrangement appears to be normal. Even after a prolonged stay in the larval hosts, proliferation processes are rare, and transdetermination was not observed.
The results are discussed, and it is suggested that the mutant effect which appears to be cell autonomous but not fully penetrant causes a mitotic disturbance which, at a low incidence, affects randomly the cell divisions of mitotically active tissues throughout larval life.
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References
Berendes, H. D.: The salivary gland chromosomes ofDrosophila hydei Sturtevant. Chromosoma (Berl.)14, 195–206 (1963)
Bryant, P. J., Schubiger, G.: Giant and duplicated imaginal discs in a new lethal mutant ofDrosophila melanogaster. Develop. Biol.24, 233–263 (1971)
Derksen, J., Berendes, H. D.: Polytene chromosome structure at the submicroscopic level. II. Length distribution of DNA molecules from polytene chromosomes ofDrosophila melanogaster andD. hydei. Chromosoma (Berl.)31, 468–477 (1970)
Ephrussi, B., Beadle, G. W.: A technique of transplantation forDrosophila. Amer. Naturalist70, 218–225 (1936)
Gehring, W.: Übertragung und Änderung der Determinationsqualitäten in Antennenscheiben-Kulturen vonDrosophila melanogaster. J. Embryol. exp. Morph.15, 77–111 (1966)
Gloor, H., Staiger, H.: Lethal-polyploid—a polyploid gene inDrosophila hydei. J. Heredity45, 289–293 (1954)
Hadorn, E.: Regulation and differentiation within field districts in imaginal discs ofDrosophila. J. Embryol. exp. Morph.1, 213–216 (1953)
Hadorn, E., Bertani, G., Gallera, J.: Regulationsfähigkeit und Feldorganisation der männlichen Genital-Imaginalscheibe vonDrosophila melanogaster. Wilhelm Roux' Arch. Entwickl.-Mech. Org.144, 31–70 (1949)
Kobel, H. R., Gloor, H.: Stock list and mutant list ofDrosophila hydei. DIS47, 39 and 47–52 (1971)
Lees, A. D., Waddington, C. H.: The development of the bristles in normal and some mutant types ofDrosophila melanogaster. Proc. Royal Soc. B131, 87–110 (1942)
Miller, A.: The internal anatomy and histology of the imago ofDrosophila melanogaster. In: Biology ofDrosophila (M. Demerec, ed.), p. 420–534. New York: John Wiley & Sons 1950
Mindek, G.: Metamorphosis of imaginal disks ofDrosophila melanogaster. Wilhelm Roux' Archiv169, 353–356 (1972)
Nöthiger, R.: The larval development of imaginal disks. In: Results and problems in cell differentiation, vol. 5 (H. Ursprung, R. Nöthiger, eds.). Berlin-Heidelberg-New York: Springer 1972
Nöthiger, R., Schubiger, G.: Developmental behaviour of fragments of symmetrical and asymmetrical imaginal discs ofDrosophila melanogaster (Diptera). J. Embryol. exp. Morph.16, 355–368 (1966)
Ouweneel, W. J.: Developmental capacities of young and mature, wildtype andopht eyeimaginal discs inDrosophila melanogaster. Wilhelm Roux' Archiv166, 76–88 (1970)
Postlethwait, J. H., Schneiderman, H. A.: A clonal analysis of development inDrosophila melanogaster: Morphogenesis, determination, and growth in the wildtype antenna. Develop. Biol.24, 477–519 (1971)
Schläpfer, T.: Der Einfluß des adulten Wirtsmilieus auf die Entwicklung von larvalen Augenantennen-Imaginalscheiben vonDrosophila melanogaster. Wilhelm Roux' Arch. Entwickl.-Mech. Org.154, 378–404 (1963)
Schubiger, G.: Anlageplan, Determinationszustand und Transdeterminationsleistungen der männlichen Vorderbeinscheibe vonDrosophila melanogaster. Wilhelm Roux' Archiv160, 9–40 (1968)
Shearn, A., Rice, T., Garen, A., Gehring, W.: Imaginal disc abnormalities in lethal mutants ofDrosophila. Proc. nat. Acad. Sci. (Wash).68, 2594–2598 (1971)
Snedecor, G. W., Cochran, W. G.: Statistical methods, 6th ed., p. 130–131, 217, 555–556. Ames, Iowa, USA: Iowa State University Press 1967
Staiger, H., Gloor, H.: Mitosehemmung und Polyploidie durch einen Letalfaktor (lpl =letal-polyploid) beiDrosophila hydei. Chromosoma (Berl.)5, 221–245 (1952)
Stewart, M., Murphy, C., Fristrom, J. W.: The recovery and preliminary characterization of X chromosome mutants affecting imaginal discs ofDrosophila melanogaster. Develop. Biol.27, 71–83 (1972)
Tobler, H.: Veränderung der Differenzierungsleistung und der Transdeterminationsfrequenz durch Colchicin in Beiminaginalscheiben vonDrosophila melanogaster. Wilhelm Roux' Archiv165, 217–225 (1970)
Ursprung, H.: Fragmentierungs- und Bestrahlungsversuche zur Bestimmung von Determinationszustand und Anlageplan der Genitalscheiben vonDrosophila melanogaster. Wilhelm Roux' Arch. Entwickl.-Mech. Org.151, 504–558 (1959)
Ursprung, H.:In vivo culture ofDrosophila imaginal discs. In: Methods in developmental biology (F. H. Wilt, N. K. Wessells, eds.), p. 485–492. New York: Thomas Y. Crowell Company 1967
Wildermuth, H., Hadorn, E.: Differenzierungsleistungen der Labialimaginalscheibe vonDrosophila melanogaster. Rev. suisse Zool.72, 686–694 (1965)
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Supported by the Fonds national suisse de la recherche scientifique; Grant No 3.224.69
The authors would like to thank Drs. H. Gloor, R. Nöthiger, D. Rungger for critical comments on this manuscript.
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Rungger-Brändle, E., van Dijk, J.P. Differentiation capacities of imaginal disks of the mutantlethal-polyploid (lpl) inDrosophila hydei . W. Roux' Archiv f. Entwicklungsmechanik 173, 333–345 (1973). https://doi.org/10.1007/BF00575838
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DOI: https://doi.org/10.1007/BF00575838