Chemistry of Natural Compounds

, Volume 19, Issue 4, pp 482–486 | Cite as

Immobilization of a protease on grafted polyethylenes

  • T. I. Davidenko
  • A. V. Chuenko
  • A. A. Bondarchuk


It has been shown that when the protease ofBacillus mesentericus is immobilized on polyethylene with grafted-on polyacrylic acid by the carbodiimide method there is a considerable retention of its activity and an increase in its thermal stability, and the immobilized preparations are active on repeated use.


Acrylic Acid Immobilize Enzyme Carbodiimide Native Enzyme Allyl Alcohol 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    T. V. Emtseva and S. A. Konovalov, Prikl. Biokhim. Mikrobiol.,14, 661 (1978).Google Scholar
  2. 2.
    T. I. Davidenko, A. V. Chuenko, and A. A. Bondarchuk, Khim. Prir. Soedin., 104 (1980).Google Scholar
  3. 3.
    O. M. Poltorak and E. S. Chukhroi, Vestn. Mosk. Univ., Ser. II, Khim.,20, 195 (1979).Google Scholar
  4. 4.
    A. Cornish-Bowden, Principles of Enzyme Kinetics, Butterworths, London (1976).Google Scholar
  5. 5.
    T. O. Galkina, A. O. Bondarchuk, and A. M. Pasichnik, Mikrobiol. Zh.,39, 286 (1977).PubMedGoogle Scholar
  6. 6.
    I. S. Petrova and T. T. Vinugonaite, Prikl. Biokhim. Mikrobiol.,2, 322 (1969).Google Scholar
  7. 7.
    T. I. Bogacheva, O. A. Mirgorodskaya, G. E. Grinberg, G. A. Mikhailets, B. V. Moskvichev, and I. I. Tereshin, Biokhimiya,46, 863 (1981).Google Scholar
  8. 8.
    E. F. Hartree, Anal. Biochem.,48, 422 (1972).CrossRefGoogle Scholar
  9. 9.
    I. V. Berezin, V. K. Antonov, and K. Martinek, Immobilized Enzymes [in Russian], Moscow, Vol. 1 (1975), p. 160.Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • T. I. Davidenko
  • A. V. Chuenko
  • A. A. Bondarchuk

There are no affiliations available

Personalised recommendations