Immobilization of a protease on grafted polyethylenes
It has been shown that when the protease ofBacillus mesentericus is immobilized on polyethylene with grafted-on polyacrylic acid by the carbodiimide method there is a considerable retention of its activity and an increase in its thermal stability, and the immobilized preparations are active on repeated use.
KeywordsAcrylic Acid Immobilize Enzyme Carbodiimide Native Enzyme Allyl Alcohol
Unable to display preview. Download preview PDF.
- 1.T. V. Emtseva and S. A. Konovalov, Prikl. Biokhim. Mikrobiol.,14, 661 (1978).Google Scholar
- 2.T. I. Davidenko, A. V. Chuenko, and A. A. Bondarchuk, Khim. Prir. Soedin., 104 (1980).Google Scholar
- 3.O. M. Poltorak and E. S. Chukhroi, Vestn. Mosk. Univ., Ser. II, Khim.,20, 195 (1979).Google Scholar
- 4.A. Cornish-Bowden, Principles of Enzyme Kinetics, Butterworths, London (1976).Google Scholar
- 6.I. S. Petrova and T. T. Vinugonaite, Prikl. Biokhim. Mikrobiol.,2, 322 (1969).Google Scholar
- 7.T. I. Bogacheva, O. A. Mirgorodskaya, G. E. Grinberg, G. A. Mikhailets, B. V. Moskvichev, and I. I. Tereshin, Biokhimiya,46, 863 (1981).Google Scholar
- 9.I. V. Berezin, V. K. Antonov, and K. Martinek, Immobilized Enzymes [in Russian], Moscow, Vol. 1 (1975), p. 160.Google Scholar