Archives of orthopaedic and traumatic surgery

, Volume 102, Issue 3, pp 179–182 | Cite as

Healing of experimental ligament rupture: Findings by scanning electron microscopy

  • O. Korkala
  • M. Rusanen
  • M. Grönblad
Original Articles

Summary

The medial collateral ligaments of the knee joints of 16 rats were cut. Sixteen ligaments were allowed to heal spontaneously while the others were immediately sutured under the microscope. Four sham-operated immobilized rats served as controls. The ligamentous healing was registered after 4–5 weeks of immobilization under a scanning electron microscope (SEM). In the control ligaments, collagen bundles of variable thickness were observed as the main supporting elements. Numerous thin fibers were seen between the bundles. The surgically corrected ligaments showed a microstructural picture often difficult to distinguish from the controls. The unsutured ligaments, however, showed a much more variable picture. In some unsutured cases ligamentous regeneration was blocked by extensive scar formation. In many unsutured cases, however, signs of ligamentous regeneration were observed. It was noted that the SEM is a useful and sophisticated tool for studies on ligament healing.

Keywords

Medial Collateral Ligament Collagen Bundle Sophisticated Tool Ligament Rupture Extensive Scar 

Zusammenfassung

Bei 16 Ratten wurde das mediale Kollateralband der Kniegelenke durchtrennt. 16 Ligamente wurden der Spontanheilung überlassen. Die anderen 16 Bänder wurden unverzüglich unter dem Operationsmikroskop genäht. Vier scheinoperierte Ratten dienten zur Kontrolle. Die Ligamente von jeder Gruppe wurden nach einer Immobilisation von 4–5 Wochen im Raster-Elektronenmikroskop untersucht: Bei den Kontroll-Ligamenten wurden hauptsächlich Kollagen-Faserbündel von variablem Durchmesser gefunden. Zwischen den Faserbündeln lagen zahlreiche dünne Fibrillen. Die operativ wiederhergestellten Bänder zeigten ein mikroskopisches Bild, welches oft schwer von den Kontrollbildern zu unterscheiden war. Die nichtgenähten Bänder hingegen zeigten ein viel bunteres Bild. In einigen Fällen war die Ligamentheilung durch eine extensive Narbenbildung blockiert. Bei vielen nichtgenähten Bändern wurde jedoch auch eine beginnende Regeneration festgestellt. Die Untersuchung im Raster-Elektronenmikroskop hat sich für experimentelle Studien der Ligament-Heilung als sehr nützlich erwiesen.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • O. Korkala
    • 1
  • M. Rusanen
    • 1
  • M. Grönblad
    • 2
  1. 1.Department of Orthopaedics and TraumatologyUniversity Central Hospital, Töölö HospitalHelsinki 26
  2. 2.Department of AnatomyUniversity of HelsinkiHelsinkiFinland

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