Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro

  • J. Benson
  • H. Oberlander
  • Mosato Koreeda
  • Koji Nakanishi
Article

Summary

In previous papers we have reported that wing disks ofGalleria mellonella complete tracheole migration and evagination when they are cultured in a chemically defined medium containing alpha-ecdysone. The presence of larval fat body in the culture medium greatly improved the response of the disks. We have isolated this fat body factor from culture medium. It is dialyzable, heat stable (100° C, 5 min), and insensitive to pronase digestion. The fat body factor, recovered from thin layer plates, stimulated evagination in the absence of alpha-ecdysone. Furthermore, radiochromatography studies with tritiated alpha-ecdysone and spectral analysis of the factor indicated that it was neither a metabolite of alpha-ecdysone nor an ecdysone-like molecule.

Keywords

Thin Layer Spectral Analysis Developmental Biology Heat Stable Wing Disk 

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References

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • J. Benson
    • 1
    • 2
  • H. Oberlander
    • 1
    • 2
  • Mosato Koreeda
    • 1
    • 2
  • Koji Nakanishi
    • 1
    • 2
  1. 1.Department of BiologyBrandeis UniversityWaltham
  2. 2.Department of ChemistryColumbia UniversityNew York

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