Chemistry of Natural Compounds

, Volume 22, Issue 2, pp 213–218 | Cite as

Comparative characteristics of methods for obtaining oligodeoxyribonucleotide 3′-phosphates

  • E. M. Volkov
  • S. M. Gryaznov
  • N. F. Krynetskaya
  • T. S. Oretskaya
  • V. K. Potapov


Methods of obtaining oligodeoxyribonucleotide 3′-phosphates based on the triester synthesis of oligodeoxyribonucleotides are discussed. These methods can be divided into two classes: I) phosphorylation of the 3′-OH group in a protected oligo-deoxyribonucleotide; and II) modification of a terminal nucleotide 3′-phosphate. It has been shown that a universal method is the preparation of an oligonucleotide with a 3′-terminal uridine residue followed by its oxidative elimination. The methods discussed in the paper have been used for obtaining a set of oligodeoxyribonucleotide 3′-phosphates required for the chemical “cross-linking” of oligonucleotides in DNA duplexes.


Phosphate Nucleotide Organic Chemistry Uridine Comparative Characteristic 
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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • E. M. Volkov
  • S. M. Gryaznov
  • N. F. Krynetskaya
  • T. S. Oretskaya
  • V. K. Potapov

There are no affiliations available

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