Advertisement

Chemistry of Natural Compounds

, Volume 20, Issue 4, pp 475–478 | Cite as

Polypeptides possessing catalytic properties

  • Sh. Kh. Khalikov
  • S. V. Alieva
  • R. V. Valiev
Article
  • 18 Downloads

Abstract

The catalytic properties of synthetic polypeptides containing L-amino acids with the sequences H-(His-Glu)n-OH, H-(Ser-Glu)n-OH, H-(His-Tyr)n-OH, and H-(Trp-Glu)n-OH in the hydrolysis of p-NPA (para-nitrophenyl acetate) are considered. The dependences of the rates of the polypeptide-catalyzed hydrolysis of p-NPA on the pH of the medium, the temperature, and the concentration of p-NPA are discussed. Vmax, Km, and K″ — the effective rate constants of the hydrolysis of p-NPA — and K2 — the constant for the splitting out of p-nitrophenol from the substrate — have been found and calculated.

Keywords

Polypeptide Chymotrypsin Cotton Seed Effective Rate Constant Synthetic Polypeptide 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    J. J. Birktoft and B. S. Hartly, Nature (London),221, 337 (1969).CrossRefGoogle Scholar
  2. 2.
    I-Nan Hsu and L. T. J. Delbare, Nature (London),267, 808 (1977).CrossRefGoogle Scholar
  3. 3.
    V. K. Naifhani, K. B. Natbun, and M. M. Dhow, Indian J. Biochem.,6, No. 1, 10 (1960).Google Scholar
  4. 4.
    M. Fridkin and H. I. Goren, Eur. J. Biochem.,39, 63 (1973).CrossRefGoogle Scholar
  5. 5.
    D. M. Blow and T. A. Steitz, Am. Rev. Biochem.,39, 63 (1973).CrossRefGoogle Scholar
  6. 6.
    D. Petz and F. Schneider, Febs. Lett.,67, 1 (1976).CrossRefGoogle Scholar
  7. 7.
    V. A. Shibnev, M. I. Ismailov, Sh. Kh. Khalikov, and I. Yu. Lakizova, Khim. Prir. Soedin., 687 (1979).Google Scholar
  8. 8.
    Sh. Kh. Khalikov, M. I. Ismailov, S. V. Alieva, and R. V. Valiev, Khim. Prir. Soedin., 91 (1982).Google Scholar
  9. 9.
    I. Photaki and V. Bardanos, J. Chem. Soc., Chem. Commun., 275 (1967).Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Sh. Kh. Khalikov
  • S. V. Alieva
  • R. V. Valiev

There are no affiliations available

Personalised recommendations