Summary
Dissociation and reassociation experimentsin vitro were carried out to investigate the differentiation potency of chick allantoic endoderm under the influence of digestive tract mesenchymes.
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1.
The allantoic endoderm, when cultured alonein vitro, shows no differentiation whatsoever.
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2.
The allantoic endoderm, when cultivated combined with the mesenchyme of oesophagus, can differentiate into a stratified cuboidal epithelium, similar to that of the embryonic oesophagus.
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3.
Cultivation of the allantoic endoderm combined with the proventricular mesenchyme causes differentiation of cylindrical epithelium and glands, which are characteristic of the embryonic proventriculus.
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4.
The combination of the allantoic endoderm and the gizzard mesenchyme results in the differentiation of pseudostratified columnar epithelium, the cells of which possess glycogen granules like those in the normal embryonic gizzard.
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5.
If the allantoic endoderm is cultured on the mesenchyme of the small intestine, the endodermal cells are converted into simple columnar epithelial cells similar to those of normal embryonic small intestine.
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6.
The competence for the heterotypic development of the allantoic endoderm appears to be a function of a developmental time sequence: it is highest in the youngest (3-day) allantoic endoderm, and gradually lost in older embryos.
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7.
In all combinations tested, there appear goblet cells in the epithelium when the explants are cultured more than 10 days. These cells are never observed in intact oesophagus, proventriculus and gizzard, whether in normal development or in culture.
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References
Bishop-Calame, S.: Étude expérimentale de l'organogenèse du système urogénital de l'embryon de Poulet. Arch. Anat. micr. Morph. exp.55, 215–309 (1966)
Bonetti, D.: L'action inductrice du derme de l'embryon de Poulet sur l'épithélium chorionique en culture d'organin vitro. C. R. Acad. Sci. (Paris)249, 1940–1941 (1959)
Dameron, F.: Étude expérimentale de l'organogenèse du poumon: nature et spécificité des interactions épithélio-mésenchymateuses. J. Embryol. exp. Morph.20, 151–167 (1968)
David, D.: L'influence de divers mésenchymes sur la différenciation de l'épithelium gastrique du fœtus de Lapin, en culturein vitro. C. R. Acad. Sci. (Paris)264, 1062–1065 (1967)
David, D.: Étude expérimentale de l'organogenèse de l'estomac chez le fœtus de Lapin. Développement normal. Localisation expérimentale de l'ébauche présomptive. Développement en culturein vitro et en greffein ovo. Wilhelm Roux' Archiv168, 304–319 (1971)
David, D.: Les relations épithélio-mésenchymateuses au cours de l'organogenèse gastrique de fœtus de Lapin. J. Embryol. exp. Morph.27, 177–197 (1972a)
David, D.: Mode d'action et propriétés des facteurs inducteurs issus du mésenchyme gastrique et des mésenchyme hétérologues. Wilhelm Roux' Archiv170, 1–12 (1972b)
Hamburger, V., Hamilton, H. L.: A series of normal stages in the development of the chick embryo. J. Morph.88, 49–92 (1951)
Kato, Y.: Epithelial metaplasia induced on extraembryonic membranes. I. Induction of epidermis from chick chorionic epithelium. J. exp. Zool.170, 229–252 (1969)
Kato, Y., Hayashi, Y.: The inductive transformation of the chorionic epithelium into skin derivatives. Exp. Cell Res.31, 599–602 (1963)
Le Douarin, N.: Étude expérimentale de l'organogenèse du tube digestif et du foie chez l'embryon de Poulet. Bull. biol. France Belg.98, 543–676 (1964)
McLoughlin, C. B.: The importance of mesenchymal factors in the differentiation of chick epidermis. II. Modification of epidermal differentiation by contact with different types of mesenchyme. J. Embryol. exp. Morph.9, 385–409 (1961)
Mizuno, T.: Induction de germes plumaires,in vitro, dans l'épithelium proamniotique de Poulet, associé au derme de la peau dorsale. C. R. Acad. Sci. (Paris)271, 2027–2030 (1970)
Mizuno, T.: Epidermal metaplasia of proamnionic epithelium induced by dorsal skin dermis in the chick embryo. J. Embryol. exp. Morph.27, 199–213 (1972)
Mizuno, T., Yasugi, S.: Différenciationin vitro de l'épithelium de l'allantoïde associé à différénts mésenchymes du tractus digestif, chez l'embryon de Poulet. C. R. Acad. Sci. (Paris)276, 1609–1612 (1973)
Moscona, A.: Squamous metaplasia and keratinization of chorionic epithelium of the chick embryo in egg and in culture. Develop. Biol.1, 1–13 (1959)
Sawyer, R. H., Abbott, U. K., Trelford, J. D.: Inductive interactions between human dermis and chick chorionic epithelium. Science175, 572–579 (1972)
Sengel, P.: Study of organogenesis by dissociation and reassociation of embryonic rudimentsin vitro. In: Organ culture (J. A. Thomas, ed.), p. 379–458. London-New York: Academic Press 1970
Sigot, M.: Induction de la formation de glycogène dans l'épithélium de proventricule par le mésenchyme de gésier chez l'embryon de Poulet. C. R. Acad. Sci. (Paris)256, 4970–4971 (1963)
Sigot, M., Marin, L.: Organogenèse de l'estomac de l'embryon de Poulet. Evolution de l'épithelium du proventricule au contact de surfaces conditionnées par des mésenchymes. J. Embryol. exp. Morph.24, 43–63 (1970)
Soriano, L.: Différenciation des épithélium du tube digestifin vitro. J. Embryol. exp. Morph.14, 119–128 (1965)
Wolff, Et., Haffen, K.: Sur une méthode de culture d'organes embryonnairesin vitro. Tex. Rep. Biol. Med.10, 463–472 (1952)
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The authors are indebted to Professor Etienne Wolff of Collège de France and Professor Emeritus Takashi Fujii of University of Tokyo for their continuous interest in this work.
This study was supported in part by a grant from the Ministry of Education of Japan.
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Yasugi, S., Mizuno, T. Heterotypic differentiation of chick allantoic endoderm under the influence of various mesenchymes of the digestive tract. W. Roux' Archiv f. Entwicklungsmechanik 174, 107–116 (1974). https://doi.org/10.1007/BF00573624
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DOI: https://doi.org/10.1007/BF00573624