Summary
1. Specific sorbents for the affinity chromatography of pepsin on hexamethylenediamine-Sepharose using valine peptides differing by the acyl groups and configurations of the valine residue have been synthesized.
2. The sorbents investigated are effective in the purification of pepsin with a low specific activity when 30–40 mg of protein is deposited on 1 ml of resin, a fourfold purification of the enzyme being achieved.
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Additional information
M. V. Lomonosov Moscow State University. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 387–392, May–June, 1977.
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Lysogorskaya, E.N., Oksenoit, E.S., Morozova, E.A. et al. Affinity chromatography of pepsin using pepstatin fragments as ligands of specific sorbents. Chem Nat Compd 13, 328–332 (1977). https://doi.org/10.1007/BF00573554
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DOI: https://doi.org/10.1007/BF00573554