Acta Diabetologica

, Volume 29, Issue 3–4, pp 237–239 | Cite as

Na+/H+ antiporter properties in peripheral blood lymphocytes from normotensive obese and type 2 diabetic patients do not differ significantly from controls

  • D. Ghigo
  • P. Alessio
  • S. Burzacca
  • C. Costamagna
  • G. Anfossi
  • F. Cavalot
  • A. Bosia
  • M. Trovati
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Abstract

It has been hypothesized that some genetic factors link different conditions characterized by the presence of insulin resistance: among them, obesity, type 2 (non-insulin-dependent) diabetes mellitus and arterial hypertension. A good candidate could be the Na+/H+ exchanger, the increased activity of which is considered a genetic marker of essential hypertension. In this study we looked at whether the Na+ dependence of the Na+/H+ antiporter is modified in obese and type 2 diabetic patients, in the absence of arterial hypertension. The activity of this ion exchanger was measured in peripheral blood lymphocytes by acidifying them in Na+-free buffer and then monitoring the recovery of intracellular pH after Na+ addition. Quiescent lymphocytes were used because they do not have insulin receptors, thus ruling out the effects of the elevated insulin concentrations on the Na+/H+ exchanger activity. Antiport activity, measured as the ability to extrude H+ in the presence of external Na+, showed no differences in normotensive obese and type 2 diabetic patients when compared with healthy subjects. Our data therefore suggest that an altered Na+/H+ exchange activity cannot be considered a common feature of insulin-resistant states.

Key words

Arterial hypertension Na+/H+ antiport Obesity Peripheral blood lymphocytes Type 2 diabetes 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • D. Ghigo
    • 1
  • P. Alessio
    • 1
  • S. Burzacca
    • 2
  • C. Costamagna
    • 1
  • G. Anfossi
    • 2
  • F. Cavalot
    • 2
  • A. Bosia
    • 1
  • M. Trovati
    • 2
  1. 1.Department of Genetics, Biology and Medical ChemistryUniversity of TurinTurinItaly
  2. 2.Department of Clinical and Biological Sciences, Diabetic UnitUniversity of TurinItaly

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