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Effect of length of aggregation time upon sorting-out behavior of cells from chick embryo tissues

  • Roland J. Lesseps
  • Gregory Glowacki
Article

Summary

The timing hypothesis of Curtis proposes that cells which go through a sequence of types of behavior at different rates sort out from one another in aggregates. In order to further test this hypothesis we have given cells from one chick embryo tissue a head start in aggregating before adding cells from a second tissue. By such experimental manipulation the normal position of cells in an aggregate should be reversed, according to predictions from the timing hypothesis. When heart ventricle cells were allowed to aggregate 6,12, 20, or 22 hours before addition of neural retina cells, the aggregates all showed internal heart cells surrounded by neural retina cells. The same final positions of heart and neural retina were found in aggregates in which neural retina cells started aggregating 4, 6, or 22 hours before addition of heart cells. Control aggregates, with heart and neural retina dissociated and co-aggregated simultaneously, also showed heart internal and neural retina external. No effect of length of aggregation time could be detected with this pair of tissues. When pigmented retina cells were allowed to aggregate 6 or 20 hours before addition of heart cells, the cells were in the same final positions as in control aggregates, namely heart external and most pigmented retina cells internal. The only position reversal occurred when heart cells were given 6 or 20 hours to aggregate before addition of pigmented retina cells, which now took up all external positions. This position reversal could result from the heart cells becoming more adhesive with time in culture.

Keywords

Retina Developmental Biology Experimental Manipulation Final Position Normal Position 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Armstrong, P. B.: Light and electron microscope studies of cell sorting in combinations of chick embryo neural retina and retinal pigment epithelium. Wilhelm Roux' Archiv168, 125–141 (1971)Google Scholar
  2. Armstrong, P. B., Niederman, R.: Reversal of tissue position after cell sorting. Develop. Biol.28, 518–527 (1972)Google Scholar
  3. Curtis, A. S. G.: Timing mechanisms in the specific adhesion of cells. Exp. Cell Res., Suppl.8, 107–122 (1961)Google Scholar
  4. Curtis, A. S. G.: Pattern and mechanism in the reaggregation of sponges. Nature (Lond.)196, 245–248 (1962)Google Scholar
  5. Curtis, A. S. G.: The cell surface: Its molecular role in morphogenesis. London: Logos Press 1967Google Scholar
  6. Curtis, A. S. G.: On the occurrence of specific adhesion between cells. J. Embryol. exp. Morph.23, 253–272 (1970)Google Scholar
  7. Lesseps, R. J.: Developmental change in morphogenetic properties: Embryonic chick heart tissue and cells segregate from other tissues in age-dependent patterns. J. exp. Zool.185, 159–168 (1973)Google Scholar
  8. Lesseps, R. J., Brown, S. A.: Further evidence for a developmental change in morphogenetic properties of embryonic chick heart cells. J. exp. Zool. in press. (1974)Google Scholar
  9. Moscona, A. A.: Rotation mediated histogenetic aggregation of dissociated cells. Exp. Cell Res.22, 455–475 (1961)Google Scholar
  10. Steinberg, M. S.: Mechanism of tissue reconstruction by dissociated cells. II: Time-course of events. Science137, 762–763 (1962)Google Scholar
  11. Steinberg, M. S.: The problem of adhesive selectivity in cellular interactions. In: Cellular membranes in development (M. Locke, ed.), p. 321–366. New York: Academic Press 1964Google Scholar
  12. Steinberg, M. S.: Does differential adhesion govern self-assembly processes in histogenesis ? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells. J. exp. Zool.173, 395–434 (1970)Google Scholar
  13. Trinkaus, J. P.: Cells into organs. The forces that shape the embryo. Englewood Cliffs, New Jersey: Prentice-Hall 1969Google Scholar
  14. Wiseman, L. L., Steinberg, M. S., Phillips, H. M.: Experimental modulation of intercellular cohesiveness: Reversal of tissue assembly patterns. Develop. Biol.28, 498–517 (1972)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Roland J. Lesseps
    • 1
  • Gregory Glowacki
    • 1
  1. 1.Department of Biological SciencesLoyola UniversityNew OrleansUSA

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