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Virus Genes

, Volume 12, Issue 2, pp 155–163 | Cite as

Molecular dissection of influenza virus RNA polymerase: PB1 subunit alone is able to catalyze RNA synthesis

  • Tetsuya Toyoda
  • Makoto Kobayashi
  • Susumu Nakada
  • Akira Ishihama
Article

Abstract

Influenza virus RNA polymerase with the subunit structure PB1-PB2-PA is involved in both transcription and replication of the RNA genome. Enzyme reconstitution experiments indicated that all three P proteins are required for RNA synthesis in vitro (Kobayashi, et al. Virus Res22, 235–245, 1992). Nuclear extracts of HeLa cells infected with three kinds of the recombinant vaccinia virus, each carrying one of the three P protein cDNAs, exhibited the activity of complete replication, that is, vRNA-sense RNA-directed and cRNA-sense RNA-directed RNA synthesis in the absence of primers. The nuclear extract from cells singly infected with the virus carrying PB1 cDNA exhibited a significant level of model v-sense RNA-directed RNA synthesis activity. Thus we conclude that PB1 is the catalytic subunit of influenza virus RNA polymerase and that under certain conditions, PB1 alone is able to catalyze RNA synthesis in vitro.

Key words

RNA polymerase influenza virus subunit function RNA replication RNA transcription recombinant vaccinia virus 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Tetsuya Toyoda
    • 1
    • 2
  • Makoto Kobayashi
    • 1
    • 3
  • Susumu Nakada
    • 4
  • Akira Ishihama
    • 1
    • 5
  1. 1.Department of Molecular GeneticsNational Institute of GeneticsMishima, ShizuokaJapan
  2. 2.Department of VirologyKurume University School of MedicineKurume, FukuokaJapan
  3. 3.Department of BiologyJichi Medical SchoolMinamikawachi, TochigiJapan
  4. 4.Molecular Medicine Research Laboratory IIIYamanouchi Pharmaceutical Co., Ltd.Tsukuba, IbarakiJapan
  5. 5.Department of Molecular GeneticsNational Institute of GeneticsMishima, ShizuokaJapan

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