Acta Diabetologica

, Volume 30, Issue 1, pp 11–16 | Cite as

Lipoprotein compositional abnormalities in type 1 (insulin-dependent) diabetic patients

  • E. Manzato
  • A. Zambon
  • S. Zambon
  • R. Nosadini
  • A. Doria
  • R. Marin
  • G. Crepaldi


Patients with type 1 (insulin-dependent) diabetes mellitus in good metabolic control usually have normal plasma lipid levels yet they have an increased incidence of vascular complications. Abnormalities in the distribution and composition of lipoprotein subfractions might in part be responsible for the macroangiopathy seen in type 1 diabetes mellitus. The plasma lipids, lipoproteins and apolipoproteins were studied in 9 type 1 diabetic patients during conventional insulin therapy and in 14 healthy controls. Plasma lipoproteins were analysed by ultracentrifugation in a zonal rotor to evaluate their concentrations and flotation properties and for compositional analysis. In diabetic patients the mean glycosylated haemoglobin (HbA1c) was 9.44±1.02% and the plasma lipid concentrations were not significantly different from healthy controls. The very low density lipoprotein (VLDL) subclass cholesterol concentrations were no different in diabetic patients and control subjects, but the VLDL cholesterol/triglyceride ratio was significantly lower in diabetic patients than in control subjects (0.34±0.05 vs 0.85±0.14; p<0.05). The flotation rate of LDL2, the major component of low density lipoprotein (LDL) was lower in the diabetic patients compared with the control subjects. The cholesterol concentrations of intermediate density lipoprotein and LDL3, the minor component of LDL, were significantly higher (0.17±0.03 and 0.83±0.14 mmol/l respectively) in diabetic patients than in control subjects (0.05±0.02 and 0.24±0.08 mmol/l). The flotation properties and cholesterol concentrations of the high density lipoprotein (HDL) subclass, and the protein-lipid composition of LDL2, HDL2 and HDL3, were no different in diabetic patients and control subjects. Diabetic patients had lower apoprotein AII and higher CII and E levels than control subjects. the plasma lipoproteins in type 1 diabetes mellitus are characterized by increased intermediate density lipoprotein and LDL3 concentrations and by abnormal LDL2 flotation properties. These lipoprotein abnormalities might have a role in atherogenesis in type 1 diabetic patients since similar alterations were associated in some recent epidemiological studies with an increased incidence of cardiovascular disease in non-diabetic patients.

Key words

Apoproteins Atherosclerosis Cholesterol Lipoproteins Triglycerides 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • E. Manzato
    • 1
  • A. Zambon
    • 1
  • S. Zambon
    • 1
  • R. Nosadini
    • 1
  • A. Doria
    • 1
  • R. Marin
    • 1
  • G. Crepaldi
    • 1
  1. 1.Department of Internal MedicineUniversity of PaduaPaduaItaly

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