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Screening fusarium strains isolated from overwintered Canadian grains for trichothecenes

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Abstract

A survey of 38 samples of Canadian overwintered grains showed that 14 (37 %) contained viableFusarium. Of a total of 38Fusarium isolates, cultured on autoclaved corn, 20 (from 7 grain samples) showed toxicity to brine shrimp larvae and 12 (from 5 samples) produced levels of trichothecenes detectable by thin layer chromatography. The principal trichothecene found was T-2 toxin, produced by 10 strains and accompanied in half of these by neosolaniol; some of these strains were identified asF. sporotrichioides Sherbakoff. Two strains ofF. poae (Peck) Wollenw. formed small amounts of diacetoxyscirpenol. T-2 toxin was the most toxic of 8 trichothecenes tested on brine shrimp larvae; the wide range of toxicities limits the usefulness of this bioassay as a general screening method for trichothecenes.

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Authors and Affiliations

  1. Food Directorate, Health Protection Branch, Health and Welfare Canada, K1A OL2, Ottawa, Ontario, Canada

    P. M. Scott, J. Harwig & B. J. Blanchfield

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  1. P. M. Scott
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  2. J. Harwig
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  3. B. J. Blanchfield
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Scott, P.M., Harwig, J. & Blanchfield, B.J. Screening fusarium strains isolated from overwintered Canadian grains for trichothecenes. Mycopathologia 72, 175–180 (1980). https://doi.org/10.1007/BF00572661

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