Summary
In psoriatic parakeratosis preserved nuclei are found in the epidermal horny layer. The question arises, whether the components of the nuclei remain unchanged. Thus we isolated and purified the DNA of psoriatic scales and normal skin. Comparing the melting points and circular dichroism of both DNAs no significant difference could be detected. These data indicate the same guaninecytosine content as well as comparabel helical compositions of the isolated DNAs.
Zusammenfassung
Bei der psoriatischen Parakeratose bleiben die Zellkerne in der Hornschicht der Epidermis erhalten. Es erhebt sich die Frage, ob die Bestandteile der Zellkerne unverändert erhalten sind. Dazu wurde sowohl aus Psoriasisschuppen als auch aus normaler menschlicher Gesamthaut die DNA isoliert und gereinigt. Beim Vergleich der Schmelzpunkte und der Circular-Dichromizität konnte kein signifikanter Unterschied festgestellt werden. Beide DNA besitzen somit den gleichen Guanin-Cytosin-Gehalt sowie vergleichbare helikale Zusammensetzung.
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Teimer, G., Ramb, G. & Leonhardi, G. Isolation and physicochemical characterisation of the DNA from normal human skin and psoriatic scales. Arch. Derm. Res. 256, 241–246 (1976). https://doi.org/10.1007/BF00572488
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DOI: https://doi.org/10.1007/BF00572488