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Heterogeneity of high-density lipoprotein particles and insulin output during oral glucose tolerance test in men with coronary artery disease

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Abstract

We compared the high-density lipoprotein (HDL) composition and particle heterogeneity in 60 non-obese (normal body mass index, BMI) men suffering from coronary artery disease (CAD) with normolipemia and normoinsulinemia with lower and higher insulin output during the oral glucose tolerance test (silent hyperinsulinemia). The apolipoprotein apoAI, apoAII, and apoE levels were higher in the high insulin response (HI) group than in low insulin response (LI) group. The ratio of apoAI versus total protein and the ratio of apoAI versus total cholesterol were increased in HI compared with LI. The lipid components in HDL were higher in HI than in HI, while for DHL2 they were higher in HI. The fractioning of HDL by gradient gel electrophoresis revealed a different pattern of HDL particles in both groups. The larger particles, HDL2b and HDL2a (mean particle diameters 10.6 and 9.2 nm, respectively), occur more frequently in HI patients (up to 60%) than in LI patients, whereas the smaller particles, HDL3a and HDL3b (mean particle diameters 8.6 and 7.8 nm, respectively), predominate in LI patients. Our results demonstrate that even in the normoglycemic, normocholesterolemic CAD patients, a high insulin output observed during the oral glucose tolerance test may be connected with a different HDL particle pattern, which suggests changes in the reverse cholesterol transport.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Kopernika str. 15A, 31-501, Cracow, Poland

    J. Iwanejko, M. Kwaśniak, I. Wybrańska, J. Hartwich, L. Guevara, A. Zdzienicka, O. Kruszelnicka-Kwiatkowska, B. Miszezuk-Jamska & A. Dembińska-Kieć

  2. Clinic of Coronary Disease, Collegium Medicum, Jagiellonian University, Cracow, Polland

    W. Piwowarska

Authors
  1. J. Iwanejko
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  2. M. Kwaśniak
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  3. I. Wybrańska
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  4. J. Hartwich
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  5. L. Guevara
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  6. A. Zdzienicka
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  7. O. Kruszelnicka-Kwiatkowska
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  8. W. Piwowarska
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  9. B. Miszezuk-Jamska
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  10. A. Dembińska-Kieć
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Iwanejko, J., Kwaśniak, M., Wybrańska, I. et al. Heterogeneity of high-density lipoprotein particles and insulin output during oral glucose tolerance test in men with coronary artery disease. Acta Diabetol 33, 58–61 (1996). https://doi.org/10.1007/BF00571944

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  • DOI: https://doi.org/10.1007/BF00571944

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