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Virus Genes

, Volume 5, Issue 1, pp 75–79 | Cite as

A novel point mutation at codon 146 of the K-ras gene in a human colorectal cancer identified by the polymerase chain reaction

  • Satoshi Orita
  • Takatsugu Higashi
  • Yasuhito Kawasaki
  • Atsuko Harada
  • Hisanaga Igarashi
  • Takushi Monden
  • Hideki Morimoto
  • Takashi Shimano
  • Takesada Mori
  • Jun Miyoshi
Article

Abstract

In this report, point mutations of the K-ras gene at codon 146 were analyzed in 25 cases of colon cancer, 4 cases of lung cancer, and 41 cases of lymphoid malignancy. A codon 146 mutation substituting threonine (ACA) for alanine (GCA) was detected in the tumor tissue of a patient with colon cancer and was not detected in the normal tissue of the same patient. Any additional mutations of theras gene family were not detected in this patient. These results suggest that the codon 146 mutation of the K-ras gene could be involved in the development of naturally occurring human malignancies.

Key words

colorectal cancer K-ras point mutation polymerase chain reaction 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Satoshi Orita
    • 1
  • Takatsugu Higashi
    • 2
  • Yasuhito Kawasaki
    • 2
  • Atsuko Harada
    • 1
  • Hisanaga Igarashi
    • 1
  • Takushi Monden
    • 2
  • Hideki Morimoto
    • 2
  • Takashi Shimano
    • 2
  • Takesada Mori
    • 2
  • Jun Miyoshi
    • 3
  1. 1.Shionogi Institute for Medical ScienceOsakaJapan
  2. 2.The Second Department of SurgeryOsaka University Medical SchoolOsakaJapan
  3. 3.Department of Oncogene Research, Research Institute for Microbial DiseasesOsaka UniversityOsakaJapan

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