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Nucleotide sequence of cDNA to the rinderpest virus mRNA encoding the nucleocapsid protein

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Abstract

The full-length cDNA corresponding to the mRNA encoding the nucleocapsid protein (NP) of rinderpest virus (RV) was cloned and its complete nucleotide sequence was determined. The gene of RV-NP was composed of 1683 nucleotides and contained a single large open reading frame, which is capable of encoding a protein of 525 amino acids with a molecular weight of 58,241 Da. The nucleotide sequence and predicted amino acid sequence were compared with those of measles virus (MV) and canine distemper virus (CDV). The nucleotide sequence of the coding region of RV-NP (53–1630) revealed a homology of 68.1% and 63.0% with MV and CDV-NP, respectively. Relatively moderate homologies of 68.7% (MV) and 64.3% (CDV) were found at nucleotides 53–592. The highest homology of 75.3–74.3% was equally present between RV and both MV and CDV in the middle region at nucleotides 593–1312. The homologies of the predicted amino acids in this region were 88.3% (MV) and 86.3% (CDV). Relatively low (MV) or little (CDV) homology was detected in the last 318 nucleotides toward the 3′ terminus (1313–1630). The predicted secondary structures of amino acids at the C terminus differed between the three viruses.

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References

  1. RimaB.K., J Gen Virol64, 1205–1219, 1983.

    Google Scholar 

  2. SatoT.A., HayamiM., and YamanouchiK., Jpn J Med Sci Biol34, 355–364, 1981.

    Google Scholar 

  3. RobbinsS.J., FenimoreJ.A., and RappF., J Gen Virol47, 301–310, 1981.

    Google Scholar 

  4. RobbinsS.J., FenimoreJ.A., and RappF., J Gen Virol48, 445–449, 1981.

    Google Scholar 

  5. FinchJ.T. and GibbsA.J., J Gen Virol6, 141–150, 1970.

    Google Scholar 

  6. SeifriedA.S., AlbrechtP., and MilstienJ.B., J Virol25, 781–787, 1978.

    Google Scholar 

  7. RozenblattS., EizenbergO., Ben-LevyR., LavieV., and WilliamJ., J Virol53, 684–694, 1985.

    Google Scholar 

  8. RozenblattS., EizenbergO., EnglundG., and WilliamJ., J Virol53, 691–694, 1985.

    Google Scholar 

  9. YoshikawaY., YamanouchiK., MorikawaY., and SakaguchiM., Microbiol Immunol26, 1195–1202, 1983.

    Google Scholar 

  10. GluzmanY., Cell23, 175–182, 1981.

    Google Scholar 

  11. IshiiH., YoshikawaY., and YamanouchiK., J Gen Virol67, 275–280, 1986.

    Google Scholar 

  12. TsukiyamaK., SugiyamaM., YoshikawaY., and YamanouchiK., Virology160, 48–54, 1987.

    Google Scholar 

  13. BilleterM.A., BackzoK., SchmidA., and terMeulenV., Virology145, 195–199, 1984.

    Google Scholar 

  14. OkayamaH. and BergP. A., Mol Cell Biol3, 280–289, 1983.

    Google Scholar 

  15. WongT.C. and HiranoA., J Virol57, 343–348, 1986.

    Google Scholar 

  16. SugiyamaM., MinamotoN., KinjoT., HirayamaN., SasakiH., YoshikawaY., and YamanouchiK., J Gen Virol70, 2605–2613, 1989.

    Google Scholar 

  17. HenikoffS., Gene28, 351–359, 1984.

    Google Scholar 

  18. Yanisch-PerronC., VieiraJ., and MessingJ., Gene33, 103–119, 1985.

    Google Scholar 

  19. MessingJ. in WuR., GrossmanL., and MoldaveK. (eds.)Methods in Enzymology, Vol. 101, Academic Press, New York, 1983, pp. 20–78.

    Google Scholar 

  20. SangerF., NicklenS., and CoulsonA.R., Proc Natl Acad Sci USA74, 5463–5467, 1977.

    Google Scholar 

  21. KyteJ. and DoolittleR.F., J Mol Biology157, 105–132, 1982.

    Google Scholar 

  22. ChouP.Y. and FasmanG.D. in MeisterH. (eds.)Advances in Enzymology, Wiley, New York, 1978, pp. 45–148.

    Google Scholar 

  23. IshidaN., TairaH., OmataT., MizumotoK., HattoriS., IwasakiK., and KawakitaM., Virus Res7, 241–255, 1987.

    Google Scholar 

  24. ShiodaT., HidakaY., KandaT., ShibutaH., NomotoA., and IwasakiK., Nucleic Acid Res11, 7317–7330, 1983.

    Google Scholar 

  25. SakaiY., SuzuS., ShiodaT., and ShibutaH., Nucleic Acid Res15, 2927–2944, 1987.

    Google Scholar 

  26. deVriesP., Versteeg van OostenJ.P.M., VisserI.K.G., vanBinnendijkR.S., LangeveldS.A., OsterhausA.D.M.E., and UysdehaagF.G.C.M., J Immunol68, 2841–2846, 1989.

    Google Scholar 

  27. van Binnedijk gnR.S., Poelen M.C.M., de Vries P., Voorma H.O., Osterhaus A.D.M.E., and Uysdehaag F.G.C.M., J Immunol142, 2837–2854.

  28. SheshberadaranH., NorrbyE., McCulloughK.C., CarterW.C., and OrvellC., J Gen Virol67, 1381–1392, 1986.

    Google Scholar 

  29. SatoT.A., FukudaA., and SugiuraA., J Gen Virol66, 1397–1409, 1985.

    Google Scholar 

  30. YamanouchiK., Jpn J Med Sci Biol33, 44–46, 1980.

    Google Scholar 

  31. OrvellC. and NorrbyE., J Gen Virol50, 231–245, 1980.

    Google Scholar 

  32. OrvellC., SheshberadaranH., and NorrbyE., J Gen Virol66, 443–456, 1985.

    Google Scholar 

  33. NorrbyE., SheshberadaranH., MulloughK.C., CarpenterW.C., and OrvellC., Intervirology23, 228–232, 1985.

    Google Scholar 

  34. TsukiyamaK., YoshikawaY., and YamanouchiK., Virology164, 523–530, 1988.

    Google Scholar 

  35. HsuD., YamanakaM., MillerJ., DaleB., GrubmanM., and YilmaT., Virology166, 149–153, 1988.

    Google Scholar 

  36. YamanakaM., HsuD., CrispT., DaleB., GrubmanM., and YilmaT., Virology166, 251–253, 1988.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan

    Hiroshi Kamata, Yasue Kamata, Yasuhiro Yoshikawa & Kazuya Yamanouchi

  2. Laboratory of Veterinary Microbiology, Nihon University Veterinary School, Fujisawa, Japan

    Hiroshi Kamata

  3. Tonen Corporation, Iruma-gun, Saitama, Japan

    Kyoko Tsukiyama

  4. Department of the Veterinary Public Health, Gifu University, Gifu, Japan

    Makoto Sugiyama

Authors
  1. Hiroshi Kamata
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  2. Kyoko Tsukiyama
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  3. Makoto Sugiyama
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  4. Yasue Kamata
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  5. Yasuhiro Yoshikawa
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  6. Kazuya Yamanouchi
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Kamata, H., Tsukiyama, K., Sugiyama, M. et al. Nucleotide sequence of cDNA to the rinderpest virus mRNA encoding the nucleocapsid protein. Virus Genes 5, 5–15 (1991). https://doi.org/10.1007/BF00571726

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  • DOI: https://doi.org/10.1007/BF00571726

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