Skip to main content
Log in

Histochemical observation of bovine spinal ganglia

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

Morphologically, large oval cells, small oval cells and intermediate cells were distinguished among the nerve cells of the bovine spinal ganglion. — The nerve cells generally showed the prominent enzymatic reactions to SDH, NAD-, NADP-dependent MDH, GDH and NAD-dependent IDH.— Large cells reacted somewhat slightly to alpha-GDH, G-6-PDH and NADP-dependent IDH, while intermediate and small cells reacted strongly. LDH reaction of large and small cells was moderate and that of intermediate cells varying. — In general, to the dehydrogenases tested, intermediate cells were most stainable and small cells reacted in the varying degrees. Considerable reactions for dehydrogenases were present in the capsular cells especially in the case of NADP-dependent IDH and G-6-PDH. The nerve fibers were less stainable to the dehydrogenases. MAO activity was observed in the capsular cells, nerve fibers and a large number of the nerve cells. — AChE reaction of the nerve cells was various and that of the capsular cells negligible, while ChE activity was limited to the capsular cells. ACP activity in nerve and capsular cells was positive, and ALP activity was confined to the capsular cells and the capillary vessels. Intense ATPase activity was localized in the capsular cells, peripheral zone of the neuroplasm and the neurilemma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arther, H.: The fine structure of young and old spinal ganglia. Anat. Rec.123, 399–423 (1955).

    Google Scholar 

  • Cayal, R. S.: Die Struktur der sensiblen Ganglien des Menschen und der Tiere. Ergebn. Anat. Entwickl.-Gesch.16, 177–215 (1906).

    Google Scholar 

  • Dixon, A. D.: Fine structure of nerve-cell bodies and satellite cells in the trigeminal ganglion. J. dent. Res.42, 990–999 (1963).

    Google Scholar 

  • Glenner, G. G., H. J. Burtner, andG. W. Brown: The histochemical demonstration of monoamine oxidase activity by tetrazolium salts. J. Histochem. Cytochem.5, 591–600 (1957).

    Google Scholar 

  • Hamberger, A.: Oxidation of tricarboxylic acid cycle intermediates by nerve cell bodies and glial cells. J. Neurochem.8, 31–35 (1961).

    Google Scholar 

  • Hokin, L. E., andM. R. Hokin: The role of phosphatidic acid and phosphoinositide in transmembrane transport elicited by acetylcholine and other humoral agents. Int. Rev. Neurobiol.2, 100–133 (1960).

    Google Scholar 

  • ——, andW. D. Shelp: The effect of acetylcholine on the turnover of phosphatidic acid and phosphoinositide in sympathetic ganglia and in various parts of the central nervous system in vitro. J. Gen. Physiol.44, 217–226 (1960).

    Google Scholar 

  • Hydén, H.: In: Biochemistry of the central nervous system. Proc. 4th. Intern. Congr. Biochem., vol. III, ed. byBrücke. New York: Pergamon Press 1959.

    Google Scholar 

  • —: The neuron and its glia-a biochemical and functional unit. Endeavour21, 144–155 (1962).

    Google Scholar 

  • —, andP. Lange: In: Regional neurochemistry, ed. byS. S. Kety andJ. Elkes. Oxford: Pergamon Press 1961.

    Google Scholar 

  • Joachim-Hermann, S. v., u.P. R. Clyde: Zur Verteilung der Kohlenhydrate und einiger Permente in Ganglion Semilunare des Rindes. Acta histochem. (Jena)5, 129–145 (1952).

    Google Scholar 

  • Katzman, R.: Electrolytic distribution in mammalian central nervous system. Neurology (Minneap.)11, 27–36 (1961).

    Google Scholar 

  • Koelle, G. B.: The histochemical identification of acetylcholinesterase in cholinergic, adrenergic and sensory neurons. J. Pharmacol. exp. Ther.114, 167–184 (1955).

    Google Scholar 

  • Korey, S. R.: Glia, lipogenesis and formation of myelin. Arch. Neurol. (Chic.)2, 141–145 (1960).

    Google Scholar 

  • Lowry, O. H.: In: Biochemistry of the developing nervous system, ed. byH. Waelsh. New York: Academic Press 1955.

    Google Scholar 

  • —: In: Metabolism of the nervous system, ed. byD. Richter. New York: Pergamon Press 1957.

    Google Scholar 

  • McDougal, D. B., E. M. Jones jr., andV. I. Sila: In: Ultrastructure and metabolism of the nervous system. Res. Publ. Ass. nerv. ment. Dis.40, 182–188 (1962).

  • Nathal, andO. Kaplan: Symposium on multiple forms of enzymes and control mechanisms. Bact. Rev.27, 155–169 (1963).

    Google Scholar 

  • Norberg, K. -A., R. Martin, andU. Urban: Histochemical study on a special cathecholamine-containing cell type in sympathetic ganglion. Acta physiol. scand.67, 260–270 (1966).

    Google Scholar 

  • Pearse, A. G. E.: In: Histochemistry, theoretical and applied. London: Churchill Ltd. 1960.

    Google Scholar 

  • Schwalbe, G.: Über den Bau der Spinalganglien, nebst Bemerkungen über die sympathischen Ganglienzellen. Arch. mikr. Anat.4, 45–72 (1868).

    Google Scholar 

  • Seto Hachiro: In: Studies on the sensory innervation. Tokyo and Osaka: Igaku Shoin 1957. [In Japanese.]

    Google Scholar 

  • Shantaveerappa, T. R., andG. H. Bourne: Histochemical studies of the distribution of dephosphorylating and oxidative enzymes and lipids in the facial and trigeminal nerves of rat. Acta anat. (Basel)51, 112–124 (1962).

    Google Scholar 

  • Spirlas, A.: Zur Kenntnis der Spinalganglienzellen der Säugetiere. Anat. Anz.11, 629–634 (1895).

    Google Scholar 

  • Tewari, H. B., andG. H. Bourne: Histochemical studies on the distribution ofβ-glucuronidase and succinate dehydrogenase in young and old spinal ganglion cells of rat. Z. Zellforsch.58, 70–75 (1962a).

    Google Scholar 

  • ——: Histochemical evidence of metabolic cycles in the spinal ganglion cells of rat. J. Histochem. Cytochem.10, 42–64 (1962b).

    Google Scholar 

  • ——: Histochemical studies on the distribution of simple esterase, specific and nonspecific cholinesterase in trigeminal ganglion cells of rat. Acta anat. (Basel)53, 319–332 (1963).

    Google Scholar 

  • ——: Histochemical studies on the distribution of alkaline and acid phosphatases and 5′-nucleotidase in the trigeminal ganglion cells of rat. Acta histochem. (Jena)17, 197–207 (1964).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsuura, H. Histochemical observation of bovine spinal ganglia. Histochemie 11, 152–160 (1967). https://doi.org/10.1007/BF00571720

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00571720

Keywords

Navigation