Advertisement

European Journal of Nuclear Medicine

, Volume 7, Issue 12, pp 531–535 | Cite as

Uptake and localization of99mTc-methylene diphosphonate in mouse osteosarcoma

  • Hiroshi Nakashima
  • Hironobu Ochi
  • Natsuo Yasui
  • Hideki Hamada
  • Keiro Ono
Article

Abstract

The localization of99mTc-methylene diphosphonate (99mTc-MDP) in mouse osteosarcoma was studied using a skeletal scintigraphic method and macro- and microautoradiographic methods. On the skeletal scintigraph, the tumor showed a high uptake of99mTc-DMP and its concentration was about 20 times as high as the value seen in the muscular tissue. The macroautoradiography showed high activity in the tumor bone region and little activity in the nonossified region. On the microautoradiography, the activity of99mTc-MDP was localized in the mineralized matrix of tumor bone and was almost nil in the nonmineralized matrix (osteoid) and the tumor cells. These findings indicate that the mineralization of the tumor bone matrix plays an important role in99mTc-MDP uptake in osteosarcoma.

Keywords

Public Health Tumor Cell High Activity Nuclear Medicine Osteosarcoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amitani K, Nakata Y, Stevens J (1974) Bone induction by lyophilized osteosarcoma in mice. Calcif Tissue Res 16:305–313Google Scholar
  2. Amitani K, Nakata Y (1977) Characteristics of osteosarcoma cells in culture. Clin Orthop 122:315–324Google Scholar
  3. Appelgren LE, Nilsson A, Ullberg S (1963) Autoradiographic localization of strontium 85 in osteosarcomas. Acta Radiol [Ther] (Stockh) 1:459–464Google Scholar
  4. Bennett HS, Wyrick AD, Lee SW, McNeil JH (1976) Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains. Stain Technol 51:71–97Google Scholar
  5. Christensen SB, Krogsgaard OW (1981) Localization of Tc-99m MDP in epiphyseal growth plates of rats. J Nucl Med 22:237–245Google Scholar
  6. Dahlin DC (1978) Bone tumors. 3rd edn. Thomas CC, Springfield, pp 226–260Google Scholar
  7. Fleisch H, Russell RGG, Francis MD (1969) Diphosphonates inhibit hydroxyapatite dissolution in vitro and bone resorption in tissue culture and in vivo. Science 165:1262–1264Google Scholar
  8. Francis MD, Russell RGG, Fleisch H (1969) Diphosphates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo. Science 165:1264–1266Google Scholar
  9. Galasko CSB (1975) The pathological basis for skeletal scintigraphy. J Bone Joint Surg [Br] 57-B:353–359Google Scholar
  10. Gerson BD, Dorfman HD, Norman A, Mankin HJ (1972) Patterns of localization of strontium 85 in osteosarcoma. J Bone Joint Surg [Am] 54-A:817–827Google Scholar
  11. Gilday DL, Ash JM, Reilly BJ (1977) Radionuclide skeletal surgery for pediatric neoplasms. Radiology 123:399–406Google Scholar
  12. Hamada H, Amitani K, Ono K (1979) Osseous alkaline phosphatase from osteosarcoma in mouse and in culture. Cell Mol Biol 25:77–84Google Scholar
  13. Huvos AG (1979) Bone tumors. Diagnosis, treatment and prognosis. W.B. Saunders, Philadelphia, pp 47–93Google Scholar
  14. Jones AG, Francis MD, Davis MA (1976) Bone scanning; Radionuclide reaction mechanisms. Semin Nucl Med 6:3–18Google Scholar
  15. Kaye M, Silverton S, Rosenthall L (1975) Technetium-99m-pyrophosphate; Studies in vivo and in vitro. J Nucl Med 16:40–45Google Scholar
  16. King MA, Casarett GW, Weber DA, Burgener FA, O'Mara RE, Wilson GA (1980) A study of irradiated bone. III. Scintigraphic and radiographic detection of radiation-induced osteosarcomas. J Nucl Med 21:426–431Google Scholar
  17. Van Langevelde A, Driessen OMJ, Pauwels EKJ, Thesingh CW (1977) Aspects of99mTechnetium binding from an ethane-1-hydroxy-1,1-diphosphonate-99mTc complex to bone. Eur J Nucl Med 2:47–51Google Scholar
  18. McNeil BJ, Cassady JR, Geiser CF, Jaffe N, Traggis D, Treves S (1973) Fluorine-18 bone scintigraphy in children with osteosarcoma or Ewing's sarcoma. Radiology 109:627–631Google Scholar
  19. Nilsson A, Ullberg S (1962). II. Uptake and retention of strontium 90 in strontium-90-induced osteosarcomas. Acta Radiol 58:168–175Google Scholar
  20. Siegel BA, Donovan RL, Alderson PO, Mack GR (1976) Skeletal uptake of99mTc-diphosphonate in relation to local bone blood flow. Radiology 120:121–123Google Scholar
  21. Simon MA, Kirchner PT (1980) Scintigraphic evaluation of primary bone tumors. J Bone Joint Surg [Am] 62-A:758–764Google Scholar
  22. Subramanian G, McAfee JG, Blair RJ, Kallfelz FA, Thomas FD (1975) Technetium-99m-methylene diphosphonate — A superior agent for skeletal imaging; Comparison with other technetium complexes. J Nucl Med 16:744–755Google Scholar
  23. Woodbury DH, Beierwaltes WH (1967) Fluorine-18 uptake and localization in soft tissue deposits of osteogenic sarcoma in rat and man. J Nucl Med 8:646–651Google Scholar
  24. Yano Y, McRae J, Van Dyke DC, Anger HO (1973) Technetium-99m-labeled stannous ethane-1-hydroxy-1,1-diphosphonate: A new bone scanning agent. J Nucl Med 14:73–78Google Scholar
  25. Zimmer AM, Isitman AT, Holmes RA (1975) Enzymatic inhibition of diphosphonates; A proposed mechnism of tissue uptake. J Nucl Med 16:352–356Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Hiroshi Nakashima
    • 1
  • Hironobu Ochi
    • 2
  • Natsuo Yasui
    • 1
  • Hideki Hamada
    • 1
  • Keiro Ono
    • 1
  1. 1.Department of Orthopaedic SurgeryOsaka University Medical SchoolOsakaJapan
  2. 2.Division of Nuclear MedicineOsaka City University Medical SchoolOsakaJapan

Personalised recommendations