European Journal of Nuclear Medicine

, Volume 7, Issue 12, pp 531–535 | Cite as

Uptake and localization of99mTc-methylene diphosphonate in mouse osteosarcoma

  • Hiroshi Nakashima
  • Hironobu Ochi
  • Natsuo Yasui
  • Hideki Hamada
  • Keiro Ono


The localization of99mTc-methylene diphosphonate (99mTc-MDP) in mouse osteosarcoma was studied using a skeletal scintigraphic method and macro- and microautoradiographic methods. On the skeletal scintigraph, the tumor showed a high uptake of99mTc-DMP and its concentration was about 20 times as high as the value seen in the muscular tissue. The macroautoradiography showed high activity in the tumor bone region and little activity in the nonossified region. On the microautoradiography, the activity of99mTc-MDP was localized in the mineralized matrix of tumor bone and was almost nil in the nonmineralized matrix (osteoid) and the tumor cells. These findings indicate that the mineralization of the tumor bone matrix plays an important role in99mTc-MDP uptake in osteosarcoma.


Public Health Tumor Cell High Activity Nuclear Medicine Osteosarcoma 
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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Hiroshi Nakashima
    • 1
  • Hironobu Ochi
    • 2
  • Natsuo Yasui
    • 1
  • Hideki Hamada
    • 1
  • Keiro Ono
    • 1
  1. 1.Department of Orthopaedic SurgeryOsaka University Medical SchoolOsakaJapan
  2. 2.Division of Nuclear MedicineOsaka City University Medical SchoolOsakaJapan

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