The ultrastructure of the liver in thyroid-fed rats

  • Unne Stenram
Article

Summary

Rats were fed on a 25% casein diet or the same diet supplemented with desiccated thyroid. The rats were killed after 16 days. Histological sections of the livers of the control rats show coarse, basophilic inclusions and abundance of glycogen in the cytoplasm. In the thyroid-fed rats there is a diffuse, cytoplasmic basophilia with basophilic rods and no or almost no glycogen. Under the electron microscope large areas of glycogen are to be seen in the cytoplasm of the control animals. Mitochondria and rough-surfaced endoplasmic membranes, often in large stacks, are found together. The liver cells of the thyroid-fed rats have little or no glycogen in their cytoplasm. Mitochondria, endoplasmic reticulum, and free ribosomes and polysomes are evenly distributed all over the cytoplasm. There seems to be an increase in the ratio of free to membrane-bound ribosomes and polysomes in the thyroid-fed rats. The possible significance of this observation in relation to RNA synthesis is discussed.

Keywords

Electron Microscope Endoplasmic Reticulum Liver Cell Control Animal Histological Section 

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References

  1. Bernhard, W.: Electron microscopy of tumor cells and tumor viruses. A review. Cancer Res.18, 491–509 (1958).Google Scholar
  2. Blobel, G., andV. Potter: Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA. J. molec. Biol.26, 279–292 (1967a).Google Scholar
  3. — —: Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes. J. molec. Biol.26, 293–301 (1967b).Google Scholar
  4. Cammarano, P., G. Giudice, andB. Lukes: Polyribosomes in regenerating livers. Biochem. biophys. Research Communic.19, 487–493 (1965).Google Scholar
  5. Campbell, P. N., G. Serck-Hanssen, andE. Lowe: Studies on the protein-synthesizing activity of the ribosomes of rat liver. The activity of free polysomes. Biochem. J.97, 422–431 (1965).Google Scholar
  6. Hallinan, T., C. N. Murty, andJ. Grant: The exclusive function of reticulum bound ribosomes in glycoprotein biosynthesis. Life sciences,7, 225–232 (1968).Google Scholar
  7. Oliver, I. T., W. F. C. Blumer, andI. J. Whitham: The ribosomes during maturation of rat liver. Comp. biochem. Physiol.10, 33–38 (1963).Google Scholar
  8. Quirin-Stricker, C., etP. Mandel: Étude du renouvellement du RNA des polysomes, du RNA transfert et du RNA „messager“ dans le foie de rat soumis à un jeune protéique. Bull. Soc. Chim. biol.50, 31–45 (1968).Google Scholar
  9. Schultz, H., H. Löw, L. Ernster undF. S. Sjöstrand: Elektronenmikroskopische Studien an Leberschnitten von Thyroxin-behandelten Ratten. In:F. S. Sjöstrand andJ. Rhodin, Electron Microscopy, Proceedings of the Stockholm Conference September 1956, p. 134–137. New York: Academic Press 1957.Google Scholar
  10. Shamoto, M.: Age difference in the ultrastructure of hepatic cells of thyroxine-treated rats. J. Gerontol.23, 1–8 (1968).Google Scholar
  11. Sidransky, H., E. Verney andH. Shinozuka: Alterations in distribution of free and membrane-bound ribosomes in the liver of rats force-fed a threonine-devoid diet. Exp. Cell. Res.54, 37–41 (1969).Google Scholar
  12. Siegel, E., andC. Tobias: Actions of thyroid hormones on cultured cells. Nature (Lond.)212 1318–1321 (1966).Google Scholar
  13. Stenram, U.: The effect of vitamin B12 and the animal protein factor on thyroid-fed rats, with special reference to liver cytology. Exp. Cell Res.3, 147–153 (1952).Google Scholar
  14. —: Basophilic rods in the liver cell cytoplasm of rat. Acta anat. (Basel)18, 360–377 (1953).Google Scholar
  15. —: Nucleolar size in the liver of rats fed on high and nonprotein diets after starvation. Acta anat. (Basel)26, 350–359 (1956).Google Scholar
  16. —: Nucleolar size in the liver of thyroid fed rats. Acta path. microbiol. scand.40, 407–412 (1957).Google Scholar
  17. —: Basophilic inclusions and rods in the liver cell cytoplasm of rat. A review. Acta histochem. (Jena)5, 156–166 (1958).Google Scholar
  18. —,H. Nordgren andR. Willén: The effect of DDT on ultrastructure and RNA and protein labelling in the liver of protein-fed and protein-deprived rats. Cytobios,1B, 51–63 (1969).Google Scholar
  19. Tata, J. R., andC. C. Widnell: Ribonucleic acid synthesis during the early action of thyroid hormones. Biochem. J.98, 604–620 (1966).Google Scholar
  20. Wannemacher, R. W., W. K. Cooper, andM. B. Yatvin: The regulation of protein synthesis in the liver of rats. Mechanisms of dietary amino acid control in the immature animal. Biochem. J.107, 615–623 (1968).Google Scholar
  21. Wunner, W. H., J. Bell andH. N. Munro: The effect of feeding with a tryptophan-free amino acid mixture on rat-liver polysomes and ribosomal ribonucleic acid. Biochem. J.101, 417–428 (1966).Google Scholar

Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • Unne Stenram
    • 1
  1. 1.Department of PathologyUniversity of UppsalaSweden

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