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Calcified Tissue International

, Volume 52, Issue 6, pp 455–459 | Cite as

PurifiedPasteurella multocida protein toxin reduces acid phosphatase-positive osteoclasts in the ventral nasal concha of gnotobiotic pigs

  • Mark R. Ackermann
  • D. A. Adams
  • L. L. Gerken
  • M. J. Beckman
  • R. B. Rimler
Laboratory Investigations

Summary

To study thein vivo response of conchal (turbinate) osteoclasts toPasteurella multocida toxin, four gnotobiotic pigs (7 days of age) were inoculated subcutaneously with 0.2 μg/kg of purified toxin. One toxin-treated pig along with one control pig were necropsied at 2, 5, 9, and 14 days postinoculation. The entire length of nasal concha from the nasal planum to ethmoid region was removed, blocked by transverse cuts into five areas, decalcified, sectioned, and then stained with tartrate-resistant acid phosphatase (TRAP) to identify osteoclasts. In each section, total area of concha, total osteoclast cytoplasmic area, and number of osteoclasts were determined using an image analysis morphometric unit. Also collected from pigs were blood and serum for complete blood counts, electrolyte levels, liver enzymes, and TRAP levels. Conchal atrophy increased in severity with time after 2 days postinoculation. In general, the ventral conchae from toxin-treated pigs at 9 and 14 days postinoculation had decreased surface area, osteoclast cytoplasmic area, and numbers of osteoclasts. Serum levels of TRAP were mildly elevated when compared with age-matched controls. No other significant alterations in blood cells or chemistries occurred and no lesions were present histologically in tissues (liver, kidney, lung, heart, and spleen) other than concha. This study shows that theP. multocida toxin induces rapid bone resorption and increases serum levels of acid phosphatase but leads to diminished acid phosphatase expression and presumably, numbers of osteoclasts.

Key words

Atrophic rhinitis Dermonecrotic toxin Tartrate-resistant acid phosphatase 

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Copyright information

© Springer-Verlag New York Inc. 1993

Authors and Affiliations

  • Mark R. Ackermann
    • 1
  • D. A. Adams
    • 2
  • L. L. Gerken
    • 2
  • M. J. Beckman
    • 3
  • R. B. Rimler
    • 1
  1. 1.Atrophic Rhinitis Project National Animal Disease Center, Agricultural Research ServiceU.S. Department of AgricultureAmesUSA
  2. 2.Department of Veterinary Anatomy, College of Veterinary MedicineIowa State UniversityAmesUSA
  3. 3.Metabolic Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research ServiceU.S. Department of AgricultureAmesUSA

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