Plant Cell Reports

, Volume 4, Issue 3, pp 168–171 | Cite as

In vitro culture of ovules ofTriticum aestivum at early stages of embryogenesis

  • M. Zenkteler
  • W. Nitzsche
Article

Abstract

Because most of the intergenericGramineae embryos abort before they can be isolated and cultured, our object was to grow ovules at an early stage of embryogenesis. Ovules were at size 1 to 7 mm. The youngest stages represented ovules containing several-celled proembryos; the oldest stage consisted of embryos at the level of differentiation of the organs. 1357 ovules were jointly inoculated in 12 different media of which only 3 appeared to be most suitable for the growth and differentiation of globular proembryos. From several-celled proembryos (ovules at the size of 1.5 – 2.5 mm) only compact calluses developed. The capability of proembryos to differentiate and to form fully developed embryos and consequently plants started when the ovules were inoculated at the size of 2.5 – 3.0 mm. Since the medium plays an important role in the process of differentiation of proembryos, the application of similar culture conditions is suggested for the in vitro culture of haploid or hybrid proembryos obtained among wide crosses inGramineae.

Keywords

Culture Condition Young Stage Similar Culture Wide Cross Compact Callus 

List of abbreviations

COC

Coconut milk

BAP

6-benzylaminopurine

YE

Yeast extract

CH

Casein hydrolysate

KT

Kinetin

ZT

Zeatin

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References

  1. Lu C, Vasil IK /1983/ Theor Appl Genet 66:285–289Google Scholar
  2. Lu C, Chandler SF, Vasil IK /1984/ J Plant Physiol 115:237–244Google Scholar
  3. Murashige T, Skoog F /1962/ Physiol Plant 15:473–497Google Scholar
  4. Norstog K, Smith J /1963/ Science 142:1655–1656Google Scholar
  5. Norstog K /1967/ Bull Torrey Bot Club 94:223–229Google Scholar
  6. Ozias-Akins F, Vasil IK /1982/ Protoplasma 110:95–105Google Scholar
  7. Rybczyński J /1979/ Genet Pol 20:11–24Google Scholar
  8. Vasil V, Vasil IK /1981/ Amer J Bot 68:864–872Google Scholar
  9. Zenkteler M, Nitzsche W /1984/ Theor Appl Genet 68: 311–315Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Zenkteler
    • 1
  • W. Nitzsche
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKöln 30FRG

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