Plant Cell Reports

, Volume 4, Issue 3, pp 115–117 | Cite as

Plant regeneration from tissue cultures initiated from immature inflorescences of a grass,Echinochloa colonum (L.) link

  • A. K. Tyagi
  • S. Bharal
  • A. Rashid
  • N. Maheshwari


Organised structures develop on a white and compact callus initiated from small segments of immature inflorescences ofEchinochloa colonum cultured on Murashige and Skoog's (MS) medium supplemented with 5.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 10% coconut milk. These develop into plantlets upon subculture onto MS medium containing 0 or 0.2 mg/l 2,4-D. Twelve out of 17 plantlets regenerated grew well on transfer to soil and eleven plants produced seeds.


Tissue Culture Organise Structure Plant Regeneration Small Segment Coconut Milk 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • A. K. Tyagi
    • 1
  • S. Bharal
    • 1
  • A. Rashid
    • 1
  • N. Maheshwari
    • 1
  1. 1.Unit for Plant Cell and Molecular Biology and Department of BotanyUniversity of DelhiDelhiIndia

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