Journal of Comparative Physiology B

, Volume 160, Issue 6, pp 691–697 | Cite as

ATP, creatine phosphate, and mechanical activity in rainbow trout myocardium under inhibition of glycolysis and cell respiration

  • T. Hartmund
  • H. Gesser


Twitch force and resting tension of electrically stimulated ventricular strips of rainbow trout were compared with tissue contents of phosphocreatine, creatine, and ATP. The phosphocreatine/total creatine ratio, which was used to assess the cytoplasmic phosphorylation potential, fell with the fraction of cell respiration that was inhibited by sodium cyanide and N2. Concomitantly, twitch force decreased while resting tension tended to increase. This relation between phosphocreatine/total creatine and mechanical parameters became more prominent as glycolysis was increasingly inhibited by sodium iodoacetate. Furthermore, glycolytic inhibition was followed by a decrease in the ATP/phosphocreatine ratio. The latter effect was the same in 1% and 6% CO2. Thus, it cannot be ascribed to an action of intracellular pH on the creatine kinase catalyzed reaction. Notably, resting tension as well as twitch force relative to ATP was augmented by glycolytic inhibition. The main conclusions are that in the presence of a decreased mitochondrial activity, glycolysis protects contractility not only by counteracting a lowering in high energy phosphates but also by supporting the ATP/phosphocreatine ratio. Apparently, the creatine kinase activity is insufficient to maintain ATP in equilibrium with phosphocreatine. In addition, glycolysis seems to elevate the level of “free” phosphate relative to ATP, so that twitch force development as well as “rigor” complex formation is counteracted.

Key words

Contractility Energy state Rainbow trout Metabolic inhibition Iodoacetae Cyanide ATP PCr 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • T. Hartmund
    • 1
  • H. Gesser
    • 1
  1. 1.Department of ZoophysiologyUniversity of AarhusAarhus CDenmark

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