Summary
The mitochondrial genomes of five rapeseed somatic hybrid plants, which combine in a first experimentBrassica napus chloroplasts and a cytoplasmic male sterility trait coming fromRaphanus sativus, and in a second experiment chloroplasts of a triazine resistantB. compestris and a cytoplasmic male sterility trait fromR. sativus, were analyzed by restriction endonucleases. Restriction fragment patterns indicate that these genomes differ from each other and from both parents. The presence of new bands in the somatic hybrid mitochondrial DNA restriction patterns is evidence of mitochondrial recombination in somatic hybrid cells. In both parental and somatic hybrid plants large quantitative variations in a mitochondrial plasmid-like DNA have been observed. Our results suggest that the cytoplasmic support for male sterility is located in the chromosomal mitochondrial DNA instead of the plasmid-like DNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility fromRaphanus sativus toBrassica oleracea. Proc Eucarpia Meeting Cruciferae, pp 52–54
Belliard G, Pelletier G, Vedel F, Quétier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids ofNicotiana tabacum. Mol Gen Genet 165:231–237
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids ofNicotiana tabacum by protoplast fusion. Nature 281:401–403
Boeshore ML, Lifshizt I, Hanson MR, Izhar S (1983) Novel composition of mitochondrial genomes inPetunia somatic hybrids derived from cytoplasmic male sterile and fertile plants. Mol Gen Genet 190:459–467
Bonnet A (1975) Introduction et utilisation d'une stérilité mâle cytoplasmique dans des variétés précoces européennes de radis (Raphanus sativus L.). Ann Amelior Plant 25:381–397
Fluhr R, Aviv D, Edelman M, Galun E (1983) Cybrids containing mixed and sorted out-chloroplasts following interspecific somatic fusions inNicotiana. Theor Appl Genet 65:289–294
Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization inNicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitution. Mol Gen Genet 186:50–56
Heyn FW (1978) Introgression of restorer genes fromRaphanus sativus into cytoplasmic male sterileBrassica napus and the genetics of fertility restoration. In: Proc 5th Int Rapeseed Conf, Malmoe, pp 82–83
Lebacq P, Vedel F (1981) Sal I restriction enzyme analysis of chloroplast and mitochondrial DNAs in the genusBrassica. Plant Sci Lett 23:1–9
Nagy F, Török I, Maliga P (1981) Extensive rearrangements in the mitochondrial DNA in somatic hybrids ofNicotiana tabacum andNicotiana knightiana. Mol Gen Genet 183:437–439
Nagy F, Lazar G, Menczel L, Maliga P (1983) A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements inNicotiana mitochondrial DNA. Theor Appl Genet 66:203–207
Ogura H (1968) Studies of the new male sterility in Japanese radish, with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6:39–78
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) An unusual mitochondrial DNA plasmid in the genusBrassica. Nature 301:725–728
Palmer JD, Shields CR (1984) Tripartite structure of theBrassica campestris mitochondrial genome. Nature 307:437–440
Pelletier G, Primard C, Vedel F, Chétrit P, Rémy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191:244–250
Schiller E, Herrmann RG, Melchers G (1982) Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol Gen Genet 186:453–459
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Vedel F, Mathieu C, Lebacq P, Ambard-Bretteville F, Rémy R, Pelletier G, Renard M, Rousselle P (1982) Comparative macromolecular analysis of the cytoplasms of normal and cytoplasmic male sterileBrassica napus. Theor Appl Genet 62:255–262
Vedel F, Mathieu C (1983) Physical and gene mapping of chloroplast DNA from normal and cytoplasmic male sterile (radish cytoplasm) lines ofBrassica napus. Curr Genet 7:13–20
Vedel F, Chétrit P, Mathieu C, Muller JP (1984) Physical and gene mapping of cauliflower (B. oleracea) mitochondrial DNA. In: 11th Katzir-Katchalsky Conf, Plant Mol Biol. Jerusalem (in press)
Author information
Authors and Affiliations
Additional information
Communicated by D. von Wettstein
Rights and permissions
About this article
Cite this article
Chetrit, P., Mathieu, C., Vedel, F. et al. Mitochondrial DNA polymorphism induced by protoplast fusion in Cruciferae. Theoret. Appl. Genetics 69, 361–366 (1985). https://doi.org/10.1007/BF00570903
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00570903