Summary
Cytophotometry was used to study the DNA content in 166 smears. The results were compared with the histological findings at cone biopsy which showed 117 carcinomas in situ, 30 microinvasive cancers, and 19 invasive carcinomas. There were statistically significant differences in DNA content and distribution between the intraepithelial neoplasia and microinvasive or invasive carcinoma. We also found cytophotometric differences between exocervical and endocervical in situ carcinoma and invasive cancers. The DNA content of cells did not vary significantly with age. Exocervical carcinoma in situ to have aneuploid histograms while endocervical in situ carcinoma had a euploid distribution of DNA, cytophotometry can help in planning the limits or extent of a cone biopsy.
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References
Andersch B, Moinian M (1982) Diagnostic and therapeutic viewpoints on cervical intraepithelial neoplasia. 10 years follow-up of a conization materia. Gynecol Obstet Invest 13: 193–205
Beato M, Castano-Almendral A, Barcellos JM (1968) Die Frühstadien des Plattenepithelcarcinoms des Collum uteri. Arch Gynecol 205: 410–427
Bennetts R, Stroehlein J, Barlogie B (1979) Ploidy determination by DNA-flow cytometry of malignant and benign gastric tissue obtained by endoscopic biopsy. Gastroenterology 76: 1099–1108
Böhm N, Sprenger E (1968) Fluorescence cytophotometry: a valuable method for the quantitative determination of nuclear feulgen DNA. Histochemie 16: 100–118
Burghardt E (1972) Entwicklung zum Zervickarzinom. In: Käser O, Friedberg V, Ober KG, Thomsen K, Zander J (eds) Gynäkologie und Geburtshilfe. Bd III. Georg-Thieme-Verlag, Stuttgart, pp 405–432
Castano-Almendral A, Müller H, Naujoks H, Castano-Almendral JL (1973) Topographical and histological localization of dysplasias, carcinoma in situ, microinvasions and microcarinomata. Gynecol Oncol 1: 320–329
Chebanu FA, Kushch AA, Zelenin AV (1977) DNA content in dysplasias and early invasive forms of carcinoma cervix uteri. Vopr Onkol 23: 64–66 (cited from ICRDB Series CB 12 No. 7)
Collste LG, Darzynkiewicz Z, Traganos F, Sharpless TK, Sogani P, Grabstald H; Whitmore WF, Melamed MR (1980) Flow cytometry in bladder cancer detection and evaluation using acridine orange metachromatic nuclei acid staining of irrigation cytology specimens. J Urol 123: 478–485
Dobreva PV, Draganov IV, Christov K, Enchev VG, Hadjiolov DH, Raichev RD (1979) DNA in the cell nucleus of mastopathia, fibroadenoma and carcinoma of the human breast. A cytophotometric study. Dokl Bolg Akad Nauk 32: 139–142 (cited from: ICRDB, Series CB 12, No. 11)
Fidler HK, Boyes, Danichols TM, Worth AJ (1970) Cervical cytology in the control of cancer of the cercix. Mod Med Can 25: 9–16
Giarelli L, Silvestri F, Antonutto G, Stanta G (1977) Observations of the pathologist on precancerous lesions of the larynx integrated with histological data and quantitative analysis of nuclear DNA content. Acta Otolaryngol Suppl 344: 7–18
Graumann W (1953) Zur Standardisierung des Schiff'schen Reagenz. Z Wiss Mikrosk 61: 225–226
Grontoft O, Hellquist H, Olofsson J, Nordstrom G (1978) The DNA content and nuclear size in normal, dysplastic and carcinomatous laryngeal epithelium. Acta Otolaryngol (Stockh) 86: 473–479
Herzog RE (1980) Cytophotometric measurement of nuclear DNA and Lysine as a method for automated screening of cervical smears. Arch Gynecol 229: 219–227
Herzog RE (1982) Cytophotometric determinations of DNA, histone, arginine, lysine and their concentrations in Eu- and Heterochromatin of the cell nucleus of dysplasias, carcinoma in situ and carcinoma of the human cervix uteri. Arch Gynecol 231: 91–98
Kimura O, Nishidoi H, Osaki Y, Miyano Y, Adachi H, Kaibara N, Koga S, Hashimoto K (1981) Comparison of leiomyoma and leiomyosarcoma on the basis of DNA histograms. Gan No Rinsho 27: 1205–1209 (cited form ICRDB, Series CB 12, No. 82/02)
Konaka C (1979) Diagnostic applications of quantitative nuclear DNA measurement of mammary tumor cells. Nippon Rinsho Saibo Gakkai Zasshi 18: 127–139 (cited from ICRDB, Series CB 12, No. 80/11)
Krug H, Ebeling K (1977) Pulse cytophotometric investigation concerning ploidy and proliferation pattern of invasive squamous cell carcinomas of the cervix uteri. Exp Pathol 13: 237–246
Morak I, Fadei L (1977) Comparative morphological and cytogenetical investigations on human ovarian granulosa cell tumors. Arch Geschwulstforsch 47: 541–549
Mukada T, Sasano N, Sato E (1978) Evaluation of esophageal dysplasia by cytofluorometric analysis Cancer 41: 1399–1404
Nishiya I, Kikuchi T, Moriya S, Shimotomai K, Sawamura I (1977) Cytophotometric study of premalignant cells of the cervix in an approach towards automated cytology. Acta Cytol 21: 271–275
Petrova AS, Zubrikhina GN, Chistiakova OV, Lukina TA (1981) DNA content in gastric mucosa of patients with various stomach lesions. Arkh Patol 43: 47–52 (cited from ICRDB, Series CB 12, No. 81/11)
Sachs H, Schittko G (1975) Zytofotometrisch ermittelte DNS-Muster in präkanzerösen Dysplasien der Cervix uteri. Beitrag zur morphologischen Abgrenzung und Klinischen Wertigkeit dieser Plattenepithel-veränderungen. Arch Gynecol 218: 95–112
Sandritter W, Jobst K, Rakow L, Bosselmann K (1963) Zur Kinetik der Feulgenreaktion bei verlängerter Hydrolysezeit. Cytophotometrische Messungen im sichtbaren und ultravioletten Licht. Histochemie 4: 420–437
Sandritter W (1964) Cytophotometrische Untersuchungen am Portiocarcinom und seinen Vorstufen. Verh Dtsch Ges Pathol 48: 34–43
Sato H, Fijiwara Y, Akiya K, Negishi Y, Umebayashi E, Yadahara Y, Hiraiwa M, Kobayashi K, Kohro J (1977) Clinical study of endometrial cancer and its border lesions, especially nucleic acid and emzymological study. Acta Obstet Gynaecol Jpn 29: 1343–1344
Sato H, Akiya K, Nakamura F, Umebayashi E, Hara S, Nutahara Y, Kobayashi K, Hiraiwa M, Mitani H, Fujiwara Y (1978) Basic study of endometrial cancer and its border disease with special reference to nuclei acid and enzyme activity. Nippon Sanka Fujinka Gakkai Zasshi 30: 1317–1323 (cited from ICRDB, Series CB 12, No. 7)
Schenck U (1979) Results of cytophotometric measurements of the DNA-content of cells from the uterine cervix. Med Klin 74: 1909–1913
Serov SF, Irzhanov SI, Bychkov VM, Zagol Skaia VN (1979) Morphogenesis of ovarian cystomas. Vopr Onkol 25: 29–36 (cited from ICRDB, Series CM 12, No. 12)
Sprenger E, Hilgarth M, Vogt-Schaden M (1978) The diagnosis of endometrial carcinoma by means of jet wash and DNA flow-through cytophotometry. Pathol Res Pract 162: 263–268
Sprenger E, Sandritter W, Naujoks H, Hilgarth M, Wagner D, Vogt-Schaden M (1977) Routine use of flow-through photometric prescreening in the detection of cervical carcinoma. Acta Cytol 21: 435–440
Sprenger E, Ulrich H, Schondorf H (1979) The diagnostic value of cell-nuclear DNA determination in aspiration citology of benign and malignant lesions of the breast. Anal Quant Cytol 1: 29–36
Sprenger E, Witte S (1980) The diagnostic significance of flow cytometric nuclear DNA measurement in gastroscopic diagnosis of the stomach. Pathol Res Pract 169: 269–275
Tsanev K, Khristova D (1979) Spectrophotometric analysis of DNA during precancer and cancer of the skin. Onkologiia 16: 114–118 (cited from ICRDB, Series CB 12, No. 80/07)
Tsanev K, Khristova D, Khristov K, Toshev I (1978) Cytophotometric analysis of DNA content in dysplasias carcinoma in situ and invasive cervical carcinoma. Onkologiia 15: 12–19
Wied GL, Messina AM, Rosenthal E (1966): Comparative quantitative DNA measurements on Feulgen-stained cervical epithelial cells. Acta Cytol 10: 31–37
Zolotareuskii VB, Popov MS (1977): Cytospectrophotometric study of the DNA content in dysplasia and mammary gland carcinoma. Arkh Patol 39: 25–31 (cited from ICRDB, Series CB 12, No. 77/08)
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Supported by Deutsche Forschungsgemeinschaft (He 781)
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Pellicer, A., Herzog, R.E. Measurement of nuclear DNA in the management of cervical intraepithelial neoplasia. Arch. Gynecol. 234, 197–204 (1984). https://doi.org/10.1007/BF00570756
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DOI: https://doi.org/10.1007/BF00570756