Abstract
We investigated different types of phosphatase activity (phosphomono-, phosphodi-, phosphotriesterase, inorganic pyrophosphatase) in five forest soils in Vorarlberg, Austria. Phosphatase activity was determined both in soils and in soil extracts prepared with different solutions (distilled water, 0.1M sodium pyrophosphate at pH 7, 0.1M sodium phosphate buffer/1M KCl at pH 6.5, and a modified universal buffer at pH 4, 6.5, 9, and 11). High phosphomonoesterase activity in these soils indicated a severe deficiency in available P. Acidic phosphomonoesterase prevailed over alkaline phosphomonoesterase activity. Phosphodiesterase was highest in the least acidic soil but no general trend towards an optimum pH was recognized. Phosphotriesterase activity was observed in only two of the five soils and favoured an alkaline optimum pH; this activity was not detected in strongly acid soils. Inorganic pyrophosphatase activity was high in soils with no phosphotriesterase. Phosphomonoesterase, phosphodiesterase and inorganic pyrophosphatase activities were much lower in soil extracts than in soils.
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References
Adams MA (1992) Phosphatase activity and phosphorus fractions in Karri (Eucalyptus diversicolor F. Muell.) forest soils. Biol Fertil Soil 14:200–204
Badalucco L, Grego S, Dell'Orco S, Nannipieri P (1992) Effect of liming on some chemical, biochemical, and microbiological properties of acid soils under spruce (Pieca abies L.). Biol Fertil Soils 14:76–83
Baligar VC, Wright RJ, Smedley MD (1988) Acid phosphatase activity in soils of the Appalachian region. Soil Sci Soc Am J 52:1612–1616
Browman MG, Tabatabai MA (1978) Phosphodiesterase activity of soils. Soil Sci Soc Am J 42:284–290
Dick WA, Tabatabai MA (1978) Inorganic pyrophosphatase activity of soils. Soil Biol Biochem 10:59–65
Dick WA, Juma NG, Tabatabai MA (1983) Effects of soils on acid phosphatase and inorganic pyrophosphatase of corn roots. Soil Sci 136:19–25
Eivazi F, Tabatabai MA (1977) Phosphatases in soils. Soil Biol Biochem 9:167–172
Gerritse RG, van Dijk H (1978) Determination of phosphatase activities of soils and animal wastes. Soil Biol Biochem 10:545–551
Gevorkyan MG, Vartanyan VT, Galystan AS, Beyleryan NM (1987) Kinetics and mechanism of action of alkaline phosphatase in an ordinary chernozem. Sov Soil Sci 19:95–100
Glatzel G, Katzensteiner K, Sieghart M (1989) Beschreibung der Versuchsflächen. In: Amt der Vorarlberger Landesregieung (ed) Lebensraum Vorarlberg, Band 3. Bregenz, pp 101–116
Grabherr G, Peter C (1989) Das Teilprojekt “Pflanzensoziologie”. In: Amt der Vorarlberger Landesregierung (ed) Lebensraum Vorarlberg, Band 3. Bregenz, pp 143–150
Harrison AF (1983) Relationship between intensity of phosphatase activity and physico-chemical properties in woodland soils. Soil Biol Biochem 15:93–99
Hayano K (1977) Extraction and properties of phosphodiesterase from a forest soil. Soil Biol Biochem 9:221–223
Hoffmann G (1968) A photometric method for the determination of the phosphatase activity in soils. Z Pflanzenernaehr Bodenkd 118:161–172
Ishii T, Hayano K (1974) A method for the estimation of phosphodiesterase activity in soil. Jpn J Soil Sci Plant Nutr 45:505–508
Juma NG, Tabatabai MA (1978) Distribution of phosphomonoesterases in soils. Soil Sci 126:101–108
Juma NG, Tabatabai MA (1988) Comparison of kinetic and thermodynamic parameters of phosphomonoesterases of soils and of corn and soybean roots. Soil Biol Biochem 20:533–539
Kandeler E (1990) Characterization of free and adsorbed phosphatases in soils. Biol Fertil Soils 9:199–202
Kishk FM, El-Essawai T, Abdel-Ghafar S, Abou-Donia MB (1976) Hydrolysis of methylparathion in soils. J Agric Food Chem 24:305–307
Malcolm RE, Vaughan D (1979) Humic substances and phosphatase activities in plant tissues. Soil Biol Biochem 11:253–259
Nakas JP, Gould WD, Klein DA (1987) Origin and expression of phosphatase activity in a semi-arid grassland soil. Soil Biol Biochem 19:13–18
Nannipieri P, Johnson RL, Paul EAB (1978) Criteria for measurement of microbial growth and activity in soil. Soil Biol Biochem 10:223–228
Nannipieri P, Ceccanti B, Cervelli S, Matarese E (1980) Extraction of phosphatase, urease, protease, organic carbon, and nitrogen from soil. Soil Sci Soc Am J 44:1011–1016
Nannipieri P, Ceccanti B, Bianchi D (1988) Characterization of humus-phosphatase complexes extracted from soil. Soil Biol Biochem 20:683–691
Pang PCK, Kolenko H (1986) Phosphomonoesterase activity in forest soils. Soil Biol Biochem 18:35–40
Parent LE, MacKenzie AF (1985) Rate of pyrophosphate hydrolysis in organic soils. Can J Soil Sci 65:497–506
Perez-Mateos M, Gonzalez-Carcedo S, Busto Nuñez D (1988) Extraction of catalase from soil. Soil Sci Soc Am J 52:408–411
Perucci P, Scarponi L, Monotti M (1988) Interference with soil phosphatase activity by maize herbicidal treatment and incorporation of maize residues. Biol Fertil Soils 6:286–291
Razzell WE, Khorana HG (1959) III Enzymatic degradation. Substrate specificity and properties of snake venom phosphodiesterase. J Biol Chem 234:2105–2113
Reid MS, Bieleski RL (1970) Changes in phosphatase activity in phosphorus-deficient Spirodela. Planta 94:713–721
Schinner F, Öhlinger R, Kandeler E, Margesin R (Hrsg) (1993) Bodenbiologische Arbeitsmethoden, 2. Auflage. Springer-Verlag, Berlin
Sparling GP, Speir TW, Whale KN (1986) Changes in microbial biomass, ATP content, soil phosphomonoesterase and phosphodiesterase activity following air-drying of soils. Soil Biol Biochem 18:363–370
Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part II chemical and microbiological properties. Am Soc Agron, Madison, Wis, pp 903–947
Tabatabai MA, Bremner JM (1969) Use ofp-nitrophenol phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307
Tabatabai MA, Fu M (1992) Extraction of enzymes from soil. Soil Biochem 7:197–227
Thornton JI, McLaren AD (1975) Enzymatic characterization of soil evidence. J. Forensic Sci 20:674–692
Trasar-Cepeda MA, Gil-Sotres F (1987) Phosphatase activity in acid high organic matter soils in Galicia (NW Spain). Soil Biol Biochem 19:281–287
Trasar-Cepeda MA, Gil-Sotres F (1988) Kinetics of acid phosphatase activity in various soils of Galicia (NW Spain). Soil Biol Biochem 20:275–280
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Margesin, R., Schinner, F. Phosphomonoesterase, phosphodiesterase, phosphotriesterase, and inorganic pyrophosphatase activities in forest soils in an alpine area: effect of pH on enzyme activity and extractability. Biol Fert Soils 18, 320–326 (1994). https://doi.org/10.1007/BF00570635
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DOI: https://doi.org/10.1007/BF00570635