Abstract
Modifiers of position-effect-variegation inDrosophila are thought to encode proteins that are either structural components of heterochromatin or enzymes that modify these components. We have recently shown that a sequence motif found in oneDrosophila modifier gene, Heterochromatin protein 1 (HP1), is conserved in a wide variety of animal and plant species (Singh et al. 1991). Using this motif, termed chromo box, we have cloned a mouse candidate modifier gene,M31, that also shows considerable sequence homology toDrosophila HP1. Here we report evidence of at least four independently segregating loci in the mouse homologous to theM31 cDNA. One of these loci—Cbx-rsl—maps to the X Chromosome (Chr), 1 cM proximal toAmg and outside the X-inactivation center region.
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References
Amar, L.C., Arnaud, D., Cambrou, J.L., Guénet, J.-L. and Avner, P.R.: Mapping of the mouse X chromosome using random genomic probes and an interspecific cross.EMBO J 4: 3695–3970, 1985.
Avner, P., Arnaud, D., Amar, L., Cambrou, J., Winking, H. and Russell, L.B.: Characterisation of a panel of somatic cell hybrids for regional mapping of the mouse X chromosome.Proc Natl Acad Sci USA 84: 5330–5334, 1987.
Brockdorff, N., Kay, G., Smith, S., Keer, J.T., Hamvas, R.M.J., Brown, S.D.M. and Rastan, S.: High-density molecular map of the central span of the mouse X chromosome.Genomics 10: 17–22, 1991.
Brown, S.D.M.: The X-inactivation center and mapping of the mouse X chromosome.In K. Davies and S. Tighman (eds.)Genome Analysis: Gene Expression and its Control, Vol. 2, Cold Spring Harbor Laboratory Press, New York, in press, 1991.
Cavanna, J.S., Coulton, G., Morgan, J.E., Brockdorff, N., Forrest, S.M., Davies, K.E. and Brown, S.D.M.: Molecular and genetic mapping of the mousemdx locus.Genomics 3: 337–341, 1988.
Chapman, V.M., Keitz, B.T., Disteche, C.M., Lau, E.C. and Snead, L.M.: Linkage of amelogenin (Amel) to the distal portion of the mouse X chromosome.Genomics 10: 23–28, 1991.
Disteche, C.M., McConnell, G.K., Grant, S.G., Stephenson, D.A., Chapman, V.M., Gandy, S. and Adler, D.A.: Comparison of the physical and recombination maps of the mouse X chromosome.Genomics 5: 177–184, 1989.
Eissenberg, J.C.: Position effect variegation inDrosophila: Towards a genetics of chromatin assembly.Bioessays 11: 14–17, 1989.
Eissenberg, J.C., James, T.C., Foster-Hartnett, D.M., Hartnett, T., Ngan, V. and Elgin, S.C.R.: Mutation in a heterochromatin-specific chromosomal protein is associated with suppressor of position-effect variegation inDrosophila melanogaster.Proc Natl Acad Sci USA 87: 9923–9927, 1991.
Irving, N.G., Hardy, J.A. and Brown, S.D.M.: The multipoint genetic mapping of mouse chromosome 16.Genomics 9: 386–389, 1991.
James, T.C. and Elgin, S.C.R.: Identification of a non-histone chromosomal protein associated with heterochromatin inDrosophila melanogaster and its gene.Mol Cell Biol 6: 3862–3872, 1986.
Keer, J.T., Hamvas, R.M.J., Brockdorff, N., Page, D., Rastan, S. and Brown, S.D.M.: Genetic mapping in the region of the mouse X-inactivation center.Genomics 7: 566–572, 1990.
Lyon, M.F.: The quest for the X-inactivation center.Trends Genet 7: 69–70, 1991.
Paro, R.: Imprinting a determined state into the chromatin ofDrosophila.Trends Genet 6: 416–421, 1990.
Paro, R. and Hogness, D.S.: The Polycomb protein shares a homologous domain with a heterochromatin-associated protein ofDrosophila.Proc Natl Acad Sci USA 88: 263–267, 1991.
Singh, P.B., Miller, J.R., Pearce, J., Kothary, R., Burton, R.D., Paro, R., James, T.C. and Gaunt, S.J.: A sequence motif found in aDrosophila heterochromatin protein is conserved in animals and plants.Nucl Acids Res 19: 789–794, 1991.
Spofford, J.: Position effect variegation inDrosophila.In M. Ashburner and E. Novitski (eds.);The Genetics and Biology of Drosophila, pp. 955–1018, Academic Press, New York, 1976.
Rastan, S. and Brown, S.D.M.: The search for the mouse X-chromosome inactivation center.Genet Res 56: 99–106, 1990.
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Hamvas, R.M.J., Reik, W., Gaunt, S.J. et al. Mapping of a mouse homolog of a Heterochromatin protein gene to the X Chromosome. Mammalian Genome 2, 72–75 (1991). https://doi.org/10.1007/BF00570443
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DOI: https://doi.org/10.1007/BF00570443