Skip to main content
SpringerLink
Log in

Enterostatin inhibits insulin secretion from isolated perifused rat islets

  • Originals
  • Published:
Acta Diabetologica Aims and scope Submit manuscript

Abstract

The effect of enterostatin on glucose-induced insulin secretion was examined in isolated, perifused rat islets. In the presence of 16.67 mM glucose, there was significant inhibition of insulin secretion at concentrations of 200 nM, 2, 20 and 40 μM enterostatin. In particular, the second phase of insulin secretion was inhibited. With a low concentration of glucose (2.78 mM), there was no significant effect on insulin secretion by enterostatin. The inhibition of insulin secretion exerted by enterostatin may be an important effect in the prevention of insulin resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Erlanson-Albertsson C, Pancreatic colipase. Structural and physiological aspects. Biochim Biophys Acta 1125:1–7, 1992

    Google Scholar 

  2. Erlanson-Albertsson C, Larsson A, A possible physiological function of procolipase activation peptide in appetite regulation. Biochimie 70:1245–1250, 1988

    Google Scholar 

  3. Erlanson-Albertsson C, Larsson A, The activation peptide of pancreatic procolipase decreases food intake in rats. Regul Pept 22:325–331, 1988

    Google Scholar 

  4. Shargill NS, Tsujii S, Bray GA, Erlanson-Albertsson C, Enterostatin suppresses food intake following injection into the third ventricle of rats. Brain Res 544:137–140, 1991

    Google Scholar 

  5. Erlanson-Albertsson C, Mei J, Okada S, York D, Bray GA, Pancreatic procolipase propeptide, enterostatin, specifically inhibitis fat intake. Physiol Behav 49:1191–1194, 1991

    Google Scholar 

  6. Okada S, York DA, Bray GA, Erlanson-Albertsson C, Enterostatin (Val-Pro-Asp-Pro-Arg) the activation peptide of procolipase selectively inhibits fat intake. Physiol Behav 49: 1185–1189, 1991

    Google Scholar 

  7. Erlanson-Albertsson C, Weström B, Pierzynowski S, Karlsson S, Ahrén B, Pancreatic procolipase activation peptide enterostatin inhibits pancreatic enzyme secretion in the pig. Pancreas 5:595–602, 1991

    Google Scholar 

  8. Gotoh M, Maki T, Satomi S, Porter J, Bonner-Weir S, O'Hara CK, Monaco AP, Reproducible high yield of rat islets by stationary in vitro digestion following pancreatic ductal or portal venous collagenase injection. Transplantation 43:725–730, 1987

    Google Scholar 

  9. Lindall A, Seffes M, Sorensen R, Immunoassayble insulin content of subcellular fractions of rat islets. Endocrinology 85:218–223, 1969

    Google Scholar 

  10. Pipeleers D, In't Veld P, Maes E, van de Winkel M, Glucose-induced insulin release depends on functional cooperation between islets cells. Proc Natl Acad Sci USA 79:7322–7325, 1982

    Google Scholar 

  11. Lacy PE, Walker MW, Fink CJ, Perifusion of isolated rat islets in vitro. Participation of the microtubular system in the biphasic release of insulin. Diabetes 21:987–998, 1972

    Google Scholar 

  12. Zimmet PZ, Collins VR, Dowse GK, Knight LT, Hyperinsulinemia in youth is a predictor of type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia 35:534–541, 1992

    Google Scholar 

  13. Le Marchand-Brustel Y, Jeanrenaud B, Pre- and postweaning studies on development of obesity in mdb/mdb mice. Am J Physiol 234:E568–E574, 1992

    Google Scholar 

  14. Le Marchand Freychet P, Jeanrenaud B, Longitudinal study on the establishment of insulin resistance in hypothalamic obese mice. Endocrinology 102:74–85, 1978

    Google Scholar 

  15. Jeanrenaud B, Halimi S, Van de Werve G, Neuro-endocrine disorders seen as triggers of the triad: obesity-insulin resistance-abnormal glucose tolerance. Diabetes Metab Rev I:261–291, 1985

    Google Scholar 

  16. Cusin I, Rohner-Jeanrenaud F, Terrettaz J, Jeanrenaud B, Insulin infusion in normal rats produces muscle insulin resistance while overstimulating white adipose tissue. In: Aihaud G, Guy-Grand B, LaFontan M, Riquier D (eds) Progress in obesity research. Libbey, London, pp 173–176, 1992

    Google Scholar 

  17. De Fronzo RA, Bonadonna RC, Ferrannini E, Pathogenesis of NIDDM. A balanced overview. Diabetes Care 15:318–368, 1992

    Google Scholar 

  18. Lillioja S, Bogardus C, Obesity and insulin resistance: lessions learned from the Pima Indians. Diabetes Metab Rev 4:515–540, 1988

    Google Scholar 

  19. Unger RH, Eisentraut AM, Entero-insular axis. Arch Intern Med 123:261–266, 1969

    Google Scholar 

  20. Creutzfeldt W, Ebert R, New developments in the incretin concept. Diabetologia 28:565–573, 1985

    Google Scholar 

  21. Örskov C, Glucagon-like peptide-1, a new hormone of the entero-insular axis. Diabetologia 35:701–711, 1992

    Google Scholar 

  22. Fletcher DR, Blackburn AM, Adrian TE, Chadwick VS, Bloom SR, Effect of neurotensin on pancreatic function in man. Life Sci 19:2157–2161, 1981

    Google Scholar 

  23. Hökfelt T, Efendic S, Hellerström C, Johansson O, Luft R, Arimura A, Cellular localization of somatostatin in endocrine-like cells and neurons of the rat with special references to A-cells of the pancreatic inslets and to the hypothalamus. Acta Endocrinol 80 [Suppl 299]:1–41, 1975

    Google Scholar 

  24. Brown JC, Vale W, Effects of neurotensin and substance P on plasma insulin, glucagon and glucose levels. Endocrinology 98:819–823, 1976

    Google Scholar 

  25. McDonald TJ, Dupré J, Tatemuto K, Greenberg GR, Radzink J, Mutt V, Galanin inhibits insulin secretion and induces hyperglycemia in dogs. Diabetes 34:192–196, 1985

    Google Scholar 

  26. Tatemoto K, Efendic S, Mutt V, Matek G, Feistner GJ, Marchas J, Pancreastatin, a novel pancreatic peptide that inhibits insulin secretion. Nature 324:476–478, 1986

    Google Scholar 

  27. Bowyer RC, Rowston WM, Jehanli AMT, Lacey JH, Hermon-Taylor J, Effect of a satiating meal on the concentrations of procolipase propeptide in the serum and urine of normal and morbidly obese subjects. Gut 34:1520–1525, 1993

    Google Scholar 

  28. Mei J, Bowyer RC, Jehanli AMT, Petel G, Erlanson-Albertsson C, Identification of enterostatin, the pancreatic procolipase activation peptide, in the intestine of rat; effect of CCK-8 and highfat feeding. Pancreas 8:488–493, 1992

    Google Scholar 

  29. Okada S, Onai T, Kilroy G, York DA, Bray GA, Adrenalectomy of the obese Zucker rat: effects on the feeding response to enterostatin and specific mRNA levels. Am J Physiol 265:R21–R27, 1993

    Google Scholar 

  30. Okada S, York DA, Bray GA, Erlanson-Albertsson C, Differential inhibition of fat intake in two strains of rat by the peptide enterostatin. Am J Physiol 262:R1111–R1116, 1992

    Google Scholar 

  31. Okada S, Lin L, York DA, Bray GA, Chronic effects of intracerebral ventricular enterostatin in Osborne-Mendel rats fed a high-fat diet. Physiol Behav 54:325–330, 1993

    Google Scholar 

  32. Olefsky JM, Insulin resistance and insulin action. An in vitro and in vivo perspective. Diabetes 30:148–162, 1981

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical and Physiological Chemistry, University of Lund, Lund, Sweden

    C. Erlanson-Albertsson

  2. Medical Clinic III and Polyclinic, Justus-Liebig University, Giessen, Germany

    B. Hering, R. G. Bretzel & K. Federlin

Authors
  1. C. Erlanson-Albertsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Hering
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. G. Bretzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Federlin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Erlanson-Albertsson, C., Hering, B., Bretzel, R.G. et al. Enterostatin inhibits insulin secretion from isolated perifused rat islets. Acta Diabetol 31, 160–163 (1994). https://doi.org/10.1007/BF00570372

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00570372

Key words

Search

Navigation