Genetic variation as a tool for histochemical localization of a nonspecific esterase
- 16 Downloads
A prominent esterase activity was demonstrated histochemically in the straight portion of the proximal tubules in kidney of the mouse strain DBA/2J after inhibition with bis-p-nitrophenyl phosphate and subsequent staining, using 5-bromoindoxyl acetate as substrate. In the strain PUC/1Fre, the corresponding esterase was only weakly expressed. By comparing data from the literature (von Deimling et al. 1981) with the characteristic features of this kidney esterase including substrate preference, sensitivity to inhibitors, solubility, histochemical location, and strain differences, it was concluded that it was identical with the previously electrophoretically defined esterase-16.
KeywordsPublic Health Phosphate Acetate Genetic Variation Characteristic Feature
Unable to display preview. Download preview PDF.
- Böcking A, Hansert J, Deimling O von (1976) Esterase XXII. Cellular and subcellular localization of the ES-9 esterase in mouse kidney. Histochemistry 46:177–188Google Scholar
- Deimling O von, Schupp P, Otto J (1981) Esterase-16 (ES-16): Characterization, polymorphism, and linkage to chromosome 3 of a kidney esterase locus of the house mouse. Biochem Genet 19:1091–1099Google Scholar
- Gomori G (1952) Histochemistry of esterase. Int Rev Cytol 1:323–335Google Scholar
- McGadey J (1967) Modified indoxyl acetate technique for the histochemical demonstration of non-specific esterases in mouse testis. J Med Lab Technol 24:126–128Google Scholar
- Maunsbach AB (1966) Observations on the segmentation of the proximal tubule in the kidney. Comparison of results from phase contrast, fluorescence and electron microscopy. J Ultrastruct Res 16:239–258Google Scholar
- Maurer HR (1971) Disc electrophoresis and related techniques of polyacrylamide gel. Electrophoresis. Walter de Gruyter, Berlin New YorkGoogle Scholar