Summary
We present a modification of Hansson's method for the demonstration of carbonic anhydrase activity. Using a semipermeable membrane together with a fluid incubation medium, frozen sections of aldehyde-fixed tissue were incubated without floating or dipping. Thin sections (thickness, 20–40 μm) were mounted on the outer surface of a tubularshaped, semipermeable cellophane dialysis membrane containing the incubation fluid. After incubation for 25–30 min at room temperature, the sections were rinsed in buffer and treated with 0.5% (NH4)2S solution. The histochemical reaction was fully inhibited by 10−4 M acetazolamide.
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Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday
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Mihály, A., Király, E., Nógrádi, A. et al. A modified semipermeable membrane technique for carbonic anhydrase histochemistry of the nervous system. Histochemistry 88, 485–487 (1988). https://doi.org/10.1007/BF00570313
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DOI: https://doi.org/10.1007/BF00570313