Human Genetics

, Volume 32, Issue 1, pp 49–64 | Cite as

Comparative studies on the specificity of anticlastogenic action in human lymphocytes in culture

  • Erich Gebhart
  • Reinhard Becher
Original Investigations

Summary

Comparative studies on human lymphocyte cultures yielded a certain specificity of the anticlastogenic action of the SH compounds 1-cysteine, cysteamine, and β-aminoethylisothiouronium (AET) as well as of the amide 1-asparagine and the amino acid 1-methionine. This specific anticlastogenic activity manifested itself in specific changes of the spectrum of aberration types induced by the clastogens and of the pattern of intercellular distribution of the induced aberrations. It was clearly dependent on the concentration of the anticlastogens but was also influenced by the used clastogen. The use of different culture media yielded some quantitative influences on the anticlastogenic activity, but fundamental changes in the spectrum of anticlastogenic action have not been observed except with cysteamine. The patterns of activity ascertained for the different anticlastogens specifically differed from those changes in the spectrum and pattern of distribution of aberrations induced by a mere reduction of the concentration for instance of Trenimon. Therefore a direct reaction between the protectors and the clastogen Trenimon as the cause of the observed anticlastogenic action was again excluded. The presented data are also discussed under the aspects of the hypotheses of aberration induction as well as of their importance for further antimutagen research.

Keywords

Internal Medicine Amide Metabolic Disease Human Lymphocyte Specific Change 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arutunian, R. M., Kuleshov, N. P.: Modification by cysteine of tio TEF induced chromosome aberrations in cultured human leucocytes. Genetika (Moscow)8, 148–153 (1972)Google Scholar
  2. Becher, R., Gebhart, E.: Die Schutzwirkung von Cysteamin und β-Aminoäthylisothiouronium (AET) gegen die chromosomenschädigende Aktivität von Treinmon® in menschlichen Lymphocyten in vitro. Humangenetik17, 307–316 (1973)Google Scholar
  3. Bender, M. A., Griggs, H. G., Bedford, J. S.: Mechanisms of chromosomal aberration production. III. Chemicals and ionizing radiation. Mutat. Res.23, 197–212 (1974)Google Scholar
  4. Dubinin, N. P., Soyfer, V. N.: Chromosome breakage and complete genic mutation production in molecular terms. Mutat. Res.8, 353–365 (1969)Google Scholar
  5. Gebhart, E.: Zur Beeinflussung der Wirkung von Myleran auf menschliche Chromosomen durch L-Cystein. Mutat. Res.7, 254–257 (1969)Google Scholar
  6. Gebhart, E.: Die Wirkung von L-Cystein auf die Aberrationsauslösung in menschlichen Chromosomen durch chemische Mutagene. I. Dosis-Wirkungs-Beziehungen bei Verwendung von Trenimon® als Aberrationsinduktor. Humangenetik10, 115–126 (1970)Google Scholar
  7. Gebhart, E.: Die Wirkung von L-Cystein auf die Aberrationsauslösung in menschlichen Chromosomen durch chemische Mutagene. II. Zur Bedeutung des Zeitfaktors bei Verwendung von Trenimon® als Aberrationsinduktor. Humangenetik11, 237–243 (1971)Google Scholar
  8. Gebhart, E.: Untersuchungen über die Schutzwirkung einiger Radioprotektoren und Aminosäuren gegen die chromosomenschädigende Aktivität chemischer Mutagene in menschlichen Lymphocyten in vitro. Habil.-Schr., Erlangen 1972Google Scholar
  9. Gebhart, E.: Untersuchungen über die Beeinflussung der chromosomenschädigenden Aktivität von Trenimon® an menschlichen Lymphocyten in vitro durch Aminosäuren. Humangenetik18, 237–246 (1973a)Google Scholar
  10. Gebhart, E.: Vergleichende Untersuchungen über die Beeinflussung der chromosomenschädigenden Aktivität von 8-Hydroxychinolinsulfat in menschlichen Lymphocyten in vitro durch Radioprotektoren und Aminosäuren. Mutat. Res.18, 353–361 (1973b)Google Scholar
  11. Gebhart, E.: Antimutagens. Data and problems. Humangenetik24, 1–32 (1974)Google Scholar
  12. Kato, H.: Induction of sister chromatid exchanges by chemical mutagens and its possible relevance to DNA repair. Exp. Cell Res.85, 239–247 (1974)Google Scholar
  13. Kline, I., Gang, M., Woodman, R. J., Venditti, J. M.: Protection with N-acetyl-1-cysteine (NSC-111180) against isophosphamide (NSC-109724) toxicity and enhancement of the therapeutic effect in early murine L 1210 leukemia. Cancer Chemother. Rep. Part. 1,57, 299–304 (1973)Google Scholar
  14. Roots, R., Okada, S.: Protection of DNA molecules of cultured mammalian cells from radiation-induced single-strand scissions by various alcohols and SH compounds. Int. J. Radiat. Biol.21, 329–342 (1972)Google Scholar
  15. Scott, D., Fox, M., Fox, B. W.: The relationship between chromosomal aberrations, survival, and DNA repair in tumour cell lines of differential sensitivity to X-rays and sulphur mustard. Mutat. Res.22, 207–221 (1974)Google Scholar
  16. Shapiro, C., Kollmann, G.: Mechanism of protection of macromolecules against ionizing radiation by sulfhydryl and other protective agents. In: Radiation damage and sulfhydryl compounds, pp. 23–43. IAEA, Vienna 1969Google Scholar
  17. Stosiek, M., Gebhart, E.: Protective effect of Reducdyn® against the chromosome damaging activity of 2,3,5-triethyleneimino-1,4-benzoquinone on human lymphocytes in vitro. Humangenetik25, 209–216 (1974)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Erich Gebhart
    • 1
  • Reinhard Becher
    • 1
    • 2
  1. 1.Institut für Humangenetik und Anthropologie der Universität Erlangen-NürnbergErlangenGermany
  2. 2.HersbruckGermany

Personalised recommendations