Summary
After centrifugation of adult quail oocytes, RNA-containing structures move in a centrifugal direction. By a moderate-speed centrifugation of quail oocytes at the end of the lampbrush or beginning postlampbrush chromosome stage, one can demonstrate that the germinal vesicle has a lower density than the surrounding ooplams. This indicates that the formation of the post-lampbrush stage and accompanying development of the germinal disc is codetermired by a gravitational (epigenetic) phenomenon. With increase in diameter of the oocyte, the cohesion between its cytoplasmic constituents progressively diminishes. The localization of the RNA-rich basophilic cortical layer after centrifugation can be followed accordingly as the oocyte grows: from a cap at the centrifugal pole in late lampbrush stage, it is progressively pushed centripetally by the peripherally formed heavy yolk that accumulates centrifugally during the postlampbrush stage.
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References
Bruce, L.: Formation of primordial yolk granules in the avian oocyte. Cell Tissue Res.178, 103–109 (1977)
Callebaut, M.: Extracorporal development of quail oocytes. Experientia24, 1242–1243 (1968)
Callebaut, M.: Extranuclear3H-thymidine incorporation in subcortical cytoplasmic organelles of primary oocytes in the Japanese quail (Coturnix coturnix japonica). Experientia26, 1134 (1970a)
Callebaut, M.: Etude histochimique des acides nucléiques dans les oocytes de la caille japonaise quelques jours avant la maturation. C.R. Soc. Biol. (Paris)164, 902–903 (1970b)
Callebaut, M.: Autoradiographic evidence for cytoplasmic DNA synthesis during the early final growth period in the oocytes of the Japanese quail. Experientia27, 305–306 (1971a)
Callebaut, M.: Incorporation de thymidine-14C dans les organites cytoplasmiques subcorticaux des oocytes de la caille japonaise quelques jours avant la maturation. C.R. Soc. Biol. (Paris)165, 2227–2229 (1971b)
Callebaut, M.: Cytoplasmic uniaxial radial symmetry during the early final growth period in the oocytes of the Japanese quail. Experientia28, 62–63 (1972)
Callebaut, M.: Correlation between germinal vesicle and oocyte development in the adult Japanese quail (Coturnix coturnix japonica). A cytochemical and autoradiographic study. J. Embryol. Exp. Morphol.29, 145–157 (1973)
Callebaut, M.: La formation de l'oocyte d'oiseau. Etude autoradiographique chez la caille japonaise pondeuse à l'aide de la leucine tritiée. Arch. Biol. (Brux.)85, 201–233 (1974)
Callebaut, M.: Bijdrage tot de studie van de oogenesis van de vogels, thesis RUCA, Antwerpen (1975)
Caro, L., Tubergen, R. van: High resolution autoradiography. J. Cell Biol.15, 173–188 (1962)
Clavert, J., Vintemberger, P.: Sur le déterminisme de la symétrie bilatérale chez les oiseaux. IV. C.R. Soc. Biol.148, 1122–1125 (1954)
Hamilton, M.G.: Ribosome structure and function. In: Methods in Enzymology,20, Nucleic acids and protein synthesis. (K. Moldave and L. Grossman, Eds.), N.Y. London: A.P. 1971
Iwamatsu, T.: Role of germinal vesicle materials on the acquisition of developmental capacity of the fish oocyte. Embryologia9, 205–221 (1966)
Kochav, Sh., Eyal-Giladi, H.: Bilateral symmetry in the chick embryo: determination by gravity. Science171, 1027–1029 (1971)
Marza, V.P., Marza, E.V.: The formation of the hen's egg. Parts I–IV. Q. J. Micr. Sci.78, 133–249 (1935)
Schjeide, O.A., Galey, F., Grellert, E.A., I-San Lin, R., De Vellis, J., Mead, I.F.: Macromolecules in oocyte maturation. Biol. Reprod. [Suppl.]2, 12–43 (1970)
Schjeide, O.A., A. Kancheva, L., Hanzely, L., Briles, W.E.: Production and fates of unique organelles (transosomes) in ovarian follicles of Gallus domesticus under various conditions. II. Cell Tissue Res.163, 63–79 (1975)
Vakaet, L.: Recherches cytologiques et cytochimiques sur l'organisation de l'oocyte I de Lebistes reticulatus. Arch. Biol.66, 1–71 (1955)
Vakaet, L.: Pregastrulatie en gastrulatie der Vogelkiem. Morphologische en Experimentele studie, Proefschrift, Arscia, Brussel (1962)
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The author is very grateful to Prof. Dr. L. Vakaet, R.U.C.A., Antwerpen, and Dr. W. Baeyens, Radiobiology S.C.K., Mol, for their valuable suggestions. The excellent technical assistance of Mrs. S. De Wolf-Van Rompaey is gratefully acknowledged.
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Callebaut, M. Effects of centrifugation on living oocytes from adult Japanese quails. Anat Embryol 153, 105–113 (1978). https://doi.org/10.1007/BF00569853
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DOI: https://doi.org/10.1007/BF00569853