Advertisement

Anatomy and Embryology

, Volume 153, Issue 1, pp 105–113 | Cite as

Effects of centrifugation on living oocytes from adult Japanese quails

  • Marc Callebaut
Article
  • 21 Downloads

Summary

After centrifugation of adult quail oocytes, RNA-containing structures move in a centrifugal direction. By a moderate-speed centrifugation of quail oocytes at the end of the lampbrush or beginning postlampbrush chromosome stage, one can demonstrate that the germinal vesicle has a lower density than the surrounding ooplams. This indicates that the formation of the post-lampbrush stage and accompanying development of the germinal disc is codetermired by a gravitational (epigenetic) phenomenon. With increase in diameter of the oocyte, the cohesion between its cytoplasmic constituents progressively diminishes. The localization of the RNA-rich basophilic cortical layer after centrifugation can be followed accordingly as the oocyte grows: from a cap at the centrifugal pole in late lampbrush stage, it is progressively pushed centripetally by the peripherally formed heavy yolk that accumulates centrifugally during the postlampbrush stage.

Key words

Oogenesis Centrifugation Japanese quail 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bruce, L.: Formation of primordial yolk granules in the avian oocyte. Cell Tissue Res.178, 103–109 (1977)Google Scholar
  2. Callebaut, M.: Extracorporal development of quail oocytes. Experientia24, 1242–1243 (1968)Google Scholar
  3. Callebaut, M.: Extranuclear3H-thymidine incorporation in subcortical cytoplasmic organelles of primary oocytes in the Japanese quail (Coturnix coturnix japonica). Experientia26, 1134 (1970a)Google Scholar
  4. Callebaut, M.: Etude histochimique des acides nucléiques dans les oocytes de la caille japonaise quelques jours avant la maturation. C.R. Soc. Biol. (Paris)164, 902–903 (1970b)Google Scholar
  5. Callebaut, M.: Autoradiographic evidence for cytoplasmic DNA synthesis during the early final growth period in the oocytes of the Japanese quail. Experientia27, 305–306 (1971a)Google Scholar
  6. Callebaut, M.: Incorporation de thymidine-14C dans les organites cytoplasmiques subcorticaux des oocytes de la caille japonaise quelques jours avant la maturation. C.R. Soc. Biol. (Paris)165, 2227–2229 (1971b)Google Scholar
  7. Callebaut, M.: Cytoplasmic uniaxial radial symmetry during the early final growth period in the oocytes of the Japanese quail. Experientia28, 62–63 (1972)Google Scholar
  8. Callebaut, M.: Correlation between germinal vesicle and oocyte development in the adult Japanese quail (Coturnix coturnix japonica). A cytochemical and autoradiographic study. J. Embryol. Exp. Morphol.29, 145–157 (1973)Google Scholar
  9. Callebaut, M.: La formation de l'oocyte d'oiseau. Etude autoradiographique chez la caille japonaise pondeuse à l'aide de la leucine tritiée. Arch. Biol. (Brux.)85, 201–233 (1974)Google Scholar
  10. Callebaut, M.: Bijdrage tot de studie van de oogenesis van de vogels, thesis RUCA, Antwerpen (1975)Google Scholar
  11. Caro, L., Tubergen, R. van: High resolution autoradiography. J. Cell Biol.15, 173–188 (1962)Google Scholar
  12. Clavert, J., Vintemberger, P.: Sur le déterminisme de la symétrie bilatérale chez les oiseaux. IV. C.R. Soc. Biol.148, 1122–1125 (1954)Google Scholar
  13. Hamilton, M.G.: Ribosome structure and function. In: Methods in Enzymology,20, Nucleic acids and protein synthesis. (K. Moldave and L. Grossman, Eds.), N.Y. London: A.P. 1971Google Scholar
  14. Iwamatsu, T.: Role of germinal vesicle materials on the acquisition of developmental capacity of the fish oocyte. Embryologia9, 205–221 (1966)Google Scholar
  15. Kochav, Sh., Eyal-Giladi, H.: Bilateral symmetry in the chick embryo: determination by gravity. Science171, 1027–1029 (1971)Google Scholar
  16. Marza, V.P., Marza, E.V.: The formation of the hen's egg. Parts I–IV. Q. J. Micr. Sci.78, 133–249 (1935)Google Scholar
  17. Schjeide, O.A., Galey, F., Grellert, E.A., I-San Lin, R., De Vellis, J., Mead, I.F.: Macromolecules in oocyte maturation. Biol. Reprod. [Suppl.]2, 12–43 (1970)Google Scholar
  18. Schjeide, O.A., A. Kancheva, L., Hanzely, L., Briles, W.E.: Production and fates of unique organelles (transosomes) in ovarian follicles of Gallus domesticus under various conditions. II. Cell Tissue Res.163, 63–79 (1975)Google Scholar
  19. Vakaet, L.: Recherches cytologiques et cytochimiques sur l'organisation de l'oocyte I de Lebistes reticulatus. Arch. Biol.66, 1–71 (1955)Google Scholar
  20. Vakaet, L.: Pregastrulatie en gastrulatie der Vogelkiem. Morphologische en Experimentele studie, Proefschrift, Arscia, Brussel (1962)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Marc Callebaut
    • 1
  1. 1.Laboratory of Anatomy and Embryology (Director: Prof. Dr. L. Vakaet)R.U.C.A.Antwerpen(Belgium)

Personalised recommendations