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Morphogenesis of the tail fiber of bacteriophage T4

III. Isolation of the gene 35-product and the genes 36, 37 and 38 product

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Summary

The formation of the tail fiber of bacteriophage T4 is controlled by genes 34, 35, 36, 37, 38 and 57. The gene 35 product was partially purified by IRC-50 column chromatography and by ammonium sulfate precipitation. The genes 36-37-38 directing component was purified 570 fold using the method of salting in and out and a sucrose density gradient centrifugation.

Some characters of the purified components and the complementation reaction between these two components were investigated.

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Author notes

  1. T. Shinomiya

    Present address: Dept. of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo, Japan

Authors and Affiliations

  1. Laboratory of Molecular Genetics, Medical School, University of Osaka, Japan

    T. Shinomiya, S. Imada & A. Tsugita

Authors
  1. T. Shinomiya
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  2. S. Imada
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  3. A. Tsugita
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Communicated by Jun-ichi Tomizawa

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Shinomiya, T., Imada, S. & Tsugita, A. Morphogenesis of the tail fiber of bacteriophage T4. Molec. Gen. Genet. 111, 368–372 (1971). https://doi.org/10.1007/BF00569789

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  • DOI: https://doi.org/10.1007/BF00569789

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