Summary
Genes encoding the enzymes cytidine deaminase (cdd), uridine monophosphate pyrophosphorylase (upp), cytidine triphosphate synthetase (pyrG), and uridine phosphorylase (udp) were located on theSalmonella typhimurium chromosome at 68, 77, 90 and 122 min, respectively. Strains carrying mutations inpyrG must also carry mutations incdd in order for cytidine to be sufficiently stable metabolically to supply the cell's requirement for CTP1.
Similar content being viewed by others
References
Adelberg, E. A., Mandel, M., Chen, G. C. C.: Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine inEscherichia coli K-12. Biochem. biophys. Res. Commun.18, 788–795 (1965).
Brockman, R. W., Davis, T. M., Stubbs, P.: Metabolism of uracil and 5-fluorouracil by drug-sensitive and by drug-resistant bacteria. Biochim. biophys. Acta (Amst.)40, 22–32 (1969).
Clark, D. J., Maaløe, O.: DNA replication and the division cycle inEscherichia coli. J. molec. Biol.23, 99–112 (1967).
Fink, G. R., Roth, J. R.: Histidine regulatory mutants inSalmonella typhimurium. J. molec. Biol.33, 547–557 (1968).
Hershey, A. D., Chan, M.: Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol.36, 39–56 (1952).
Neuhard, J.: Pyrimidine nucleotide metabolism and pathways of thymidine triphosphate biosynthesis inSalmonella typhimurium. J. Bact.96, 1519–1527 (1968).
—: Mutants ofSalmonella typhimurium requiring cytidine for growth. J. Bact.95, 2431–2433 (1968).
Newell, P. C., Tucker, R. G.: Biosynthesis of the pyrimidine moiety of thiamine. Biochem. J.106, 279–287 (1968).
Norkin, L. C.: Marker specific effects in genetic recombination. J. molec. Biol.51, 633–655 (1970).
Okada, T., Homma, F., Sonohara, H.: Improved method for obtaining thymineless mutants ofEscherichia coli andSalmonella typhimurium. J. Bact.84, 602–603 (1962).
Pittard, J., Walker, E. M.: Conjugation inEscherichia coli. Recombination events in terminal regions of transferred deoxyribonucleic acid. J. Bact.94, 1656–1663 (1967).
Roth, J. R., Antón, D. N., Hartman, P. E.: Histidine regulatory mutants inSalmonella typhimurium. J. molec. Biol.22, 305–323 (1966).
—, Hartman, P. E.: Heterogeneity in P22 transducing particles. Virology27, 297–307 (1965).
Sanderson, K. E.: Current linkage map ofSalmonella typhimurium. Bact. Rev.34, 176–193 (1970).
Smith, H. O., Levine, M.: A phage P22 gene controlling integration of prophage. Virology31, 207–216 (1967).
Author information
Authors and Affiliations
Additional information
Communicated by W. Maas
Rights and permissions
About this article
Cite this article
Beck, C.F., Ingraham, J.L. Location on the chromosome ofSalmonella typhimurium of genes governing pyrimidine metabolism. Molec. Gen. Genet. 111, 303–316 (1971). https://doi.org/10.1007/BF00569782
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00569782