Skip to main content
SpringerLink
Log in

Genetic variants of placental alkaline phosphatase as detected by a monoclonal antibody

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

Human placental alkaline phosphatase (PLAP) is a highly polymorphic enzyme. Several common as well as rare allelic forms of PLAP are characterized in this paper in terms of their reactivity with a murine monoclonal antibody (F11). The common type 1 (S) and 3 (I) variants, and the rare type 4 (S2) and 18 (D) variants were found to react with the F11 antibody, so as did three new electrophoretically defined variants (19, 20, and 21). In contrast, the common type 2 (F1) variant and the rare type 8 (F3) and 9 (F2) variants do not react with the F11 antibody. This selective reactivity of F11 has also allowed the identification of two molecular variants of PLAP with identical electrophoretic mobility. These results establish monoclonal antibodies as invaluable adjuncts in the study of PLAP polymorphism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beckman L, Beckman G (1968) A genetic variant of placental alkaline phosphatase with unusual electrophoretic properties. Acta Genet (Basel) 18:543–552

    Google Scholar 

  • Beckman G, Beckman L (1969) The placental alkaline phosphatase polymorphism: variations in Hawaiian subpopulations. Hum Hered 19:524–529

    Google Scholar 

  • Beckman L, Björling G, Christodoulou G (1966) Pregnancy enzymes and plancental polymorphism. I. Alkaline phosphatase. Acta Genet (Basel) 16:59

    Google Scholar 

  • Doellgast GJ, Kennedy M, Donald LJ (1980) The “18” variant of human placental alkaline phosphatase is identical to the “D variant”. Hum Hered 30:18–20

    Google Scholar 

  • Donald LJ, Robson EB (1974) Rare variants of placental alkaline phosphatase. Ann Hum Genet 37:303–313

    Google Scholar 

  • Engvall E (1980) Enzyme immunoassay-ELISA and EMIT. Methods Enzymol 70:419–439

    Google Scholar 

  • Fishman WH (1974) Perspectives on alkaline phosphatases. Am J Med 56:617–650

    Google Scholar 

  • Fishman L, Migayama H, Driscoll SG, Fishman WH (1976) Developmental phase-specific alkaline phosphatase isoenzyme of human placenta and their occurrence in human cancer. Cancer Res 36:2268–2273

    Google Scholar 

  • Harris H (1980) The principles of human biochemical genetics. Third revised edition. Elsevier/North-Holland, Biomedical Press, The Netherlands

    Google Scholar 

  • Holmgren PÅ, Stigbrand T (1976) Purification and partial characterization of two genetic variants of placental alkaline phosphatase. Biochem Genet 14:777–789

    Google Scholar 

  • Holmgren PÅ, Stigbrand T, Damber MG, Schoultz B von (1978) A double antibody solid phase radioimmunoassay for placental alkaline phosphatase. Clin Chim Acta 83:205–210

    Google Scholar 

  • Kearny JF, Radbruch A, Liesegang B, Rajewsky K (1979) A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol 123:1548–1550

    Google Scholar 

  • Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497

    Google Scholar 

  • Lehmann FG (1975) Immunological relationship between human placental and intestinal alkaline phosphatase. Clin Chim Acta 65: 257–269

    Google Scholar 

  • Millán JL, Stigbrand T (1981) “Sandwich” enzyme immunoassay for placental alkaline phosphatase. Clin Chem 27/12:2014–2018

    Google Scholar 

  • Millán JL, Stigbrand T, Ruoslahti E, Fishman WH (1982) Characterization of an allotype-specific monoclonal antibody to placental alkaline phosphatase and demonstration of genetic polymorphism in tumor-derived phosphatases. Cancer Res (in press)

  • Robson EB, Harris H (1967) Further studies on the genetics of placental alkaline phosphatase. Ann Hum Genet 30:219–232

    Google Scholar 

  • Slaughter CA, Coseo MC, Cancro MP, Harris H (1981) Detection of enzyme polymorphism by using monoclonal antibodies. Proc Natl Acad Sci (USA) 78:1124–1128

    Google Scholar 

  • Stigbrand T, Millán JL, Fishman WH (1982) the genetic basis of alkaline phosphatase isoenzyme expression. Isozymes: Current topics in biological and medical research, vol 6. Alan R Liss, Inc, New York (in press)

    Google Scholar 

  • Sussman HH, Gottlieb AJ (1969) Human placental alkaline phosphatase. II. Molecular and subunit properties of the enzyme. Biochim Biophys Acta 194:170–179

    Google Scholar 

  • Wei SC, Doellgast GJ (1980) Specificity of allozyme-absorbed antisera to human placental alkaline phosphatase for individual phenotypes. Biochem Genet 18:1097–1107

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiological Chemistry, University of Umeå, S-901 87, Umeå, Sweden

    José Luis Millán, Annika Jeppsson & Torgny Stigbrand

  2. Department of Medical Genetics, University of Umeå, S-901 87, Umeå, Sweden

    Gunhild Beckman

Authors
  1. José Luis Millán
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gunhild Beckman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Annika Jeppsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Torgny Stigbrand
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Millán, J.L., Beckman, G., Jeppsson, A. et al. Genetic variants of placental alkaline phosphatase as detected by a monoclonal antibody. Hum Genet 60, 145–149 (1982). https://doi.org/10.1007/BF00569701

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00569701

Keywords

Search

Navigation