Chemistry of Natural Compounds

, Volume 13, Issue 5, pp 575–577 | Cite as

The esterase and peptidase activities of an elastolytic enzyme of Actinomyces rimosus

  • L. R. Radzhabov
  • V. A. Shibiev
  • Yu. A. Rassulin
Article
  • 28 Downloads

Summary

1. It has been shown that the values of Km for the esterase activity of elastase ofActinomyces rimosus and for pancreatic elastase are of the same order of magnitude.

2. The elastase ofActinomyces rimosus, unlike the pancreatic enzyme, is capable of hydrolyzing only the methyl ester of N-acetyltrialanine, and not the mono and di derivatives.

3. It has been found that in the initial stage of the hydrolysis reaction it is mainly the ester bonds that are cleaved.

Keywords

Methyl Ester Peptide Bond Esterase Activity Thionyl Chloride Remazol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    Yu. A. Rassulin, L. R. Radzhabov, E. D. Kaverzneva, V. A. Shibnev, and G. S. Erkomaishvili, Izv. Akad. Nauk SSSR, Ser. Khim., No. 3, 687 (1974).Google Scholar
  2. 2.
    L. R. Radzhabov, Yu. A. Rassulin, L. I. Mar'yash, and V. A. Shibnev, Bioorg. Khim.,1, 554 (1975).Google Scholar
  3. 3.
    D. M. Shotton, Methods in Enzymology,19, 113 (1970).CrossRefGoogle Scholar
  4. 4.
    A. Gertler and T. Hofman, Can. J. Biochem.,51, 121 (1973).CrossRefGoogle Scholar
  5. 5.
    A. Gertler and T. Hofman, Can. J. Biochem.,48, 384 (1970).CrossRefGoogle Scholar
  6. 6.
    A. Gertler and M. Trop, Europ. J. Biochem.,19, 90 (1973).CrossRefGoogle Scholar
  7. 7.
    J. Bieth and J. Meyer, Anal. Biochem.,51, 121 (1973).CrossRefGoogle Scholar
  8. 8.
    S. Hestrin, J. Biol. Chem.,180, 249 (1949).PubMedPubMedCentralGoogle Scholar
  9. 9.
    A. P. Ryle and R. R. Porter, Biochem. J.,73, 75 (1959).CrossRefGoogle Scholar
  10. 10.
    H. Lineweaver and D. Burk, J. Am. Chem. Soc.,56, 658 (1934).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • L. R. Radzhabov
  • V. A. Shibiev
  • Yu. A. Rassulin

There are no affiliations available

Personalised recommendations