Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 311, Issue 3, pp 273–284 | Cite as

Transfer across mucosal epithelium, tissue content and metabolic fate of125I-(ipodate-sodium) on isolated everted segments of rat small intestine

  • B. Komp
  • W. Forth


  1. 1.

    Transfer and tissue content of125I-radioactivity was measured after administration of125I-(ipodate-sodium) to everted rat jejunal segments.

  2. 2.

    After having administered 10−5M125I-(ipodatesodium) on both sides of the everted sacs the S/M ratio of the concentration of125I-radioactivity was 1.5 in jejunal segments and 2.3 in ileal segments. The tissue content was nearly equal for both segments. According to the apparent partition coefficient for ipodate-sodium at pH7, the125I-radioactivity is accumulated in the tissue about 10-fold.

  3. 3.

    Lowering of the temperature of the incubation medium from 37°C to 15°C prevents the building up of a concentration gradient between the serosal and the mucosal side on either jejunal and ileal segments whereas the tissue content of125I-radioactivity was nearly unchanged.

  4. 4.

    With increasing concentrations (1.6·10−6–9.6·10−4 M) of125I-(ipodate-sodium) administered on the mucosal side the transfer and the tissue content of125I-radioactivity were decreased. This appears to be a toxic effect since in jejunal segments also the S/M ratio for the concentration of glucose decreases.

  5. 5.

    The analysis of the125I-radioactivity in the serosal fluid of jejunal segments showed that the bulk of the125I-radioactivity was present in the aqueous phase and only 33% as the unchanged ipodate-sodium in the organic phase. 10% of the125I-radioactivity must be attributed to inorganic iodine. The concentration of125I-(ipodate-sodium) administered in the mucosal fluid only was 3.2·10−6 M. At lower temperature (7°C) the bulk of the125I-radioactivity in the serosal fluid was found in the organic phase, i.e. as unchanged ipodate-sodium.

  6. 6.

    After the incubation of the aqueous phase with β-glucuronidase or NaOH about 97% of the125I-radioactivity could be extracted into the organic phase. This means that the bulk of the125I-radioactivity in the aqueous phase is present as a conjugate, e.g. ester glucuronide of the unchanged ipodate.

  7. 7.

    Apparently, the process of the conjugation of ipodate-sodium in the mucosal cells is involved in the transfer of the125I-radioactivity across the mucosal epithelium.


Key words

125I-(ipodate-sodium) Glucuronidation in the mucosal epithelium Transfer across mucosal epithelium Tissue content Metabolic conversion of ipodate-sodium during absorption 


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • B. Komp
    • 1
  • W. Forth
    • 1
  1. 1.Institut für Pharmakologie und Toxikologie der Ruhr-Universität BochumBochum 1Federal Republic of Germany

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