Summary
The frequencies of chromosome lesions were determined on 3537 mitoses in samples of varying sizes from cultures of 25 amniotic fluid specimens taken from patients at cytogenetic risk. The average percentage values of aberrant cells, including and excluding gaps, were 12.5 and 4.9, respectively. The corresponding values for fibroblasts and peripheral blood lymphocytes from normal adult donors, calculated under the same laboratory conditions, were 5.0 (including gaps) and 2.4 (excluding gaps) and 2.4 (including gaps) and 1.0 (excluding gaps), respectively. The hypothesis of a correlation between the increased incidence of chromosome lesions and the occurrence of abnormal karyotypes in amniotic fluid cell cultures is discussed.
Similar content being viewed by others
References
Boué, A., Nicolesco, H., Ravisé, N., Boué, J.: La culture des cellules du liquide amniotique. Experience de 750 cultures. Prenatal diagnosis/Diagnostic prenatal61, 69–79 (1976)
Cox, D. M., Niewczas-Late, V., Riffel, M. L., Hamerton, J. L.: Chromosomal mosaicism in diagnostic amniotic fluid cell cultures. Pediatr. Res.8, 679–683 (1974)
Evans, H. J., O'Riordan, M. L.: Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. Mutat. Res.31, 135–148 (1975)
Higurashi, M., Conen, P. E.: Comparison of chromosomal behaviour in cultured lymphocytes and fibroblasts from patients with chromosomal disorders and controls. Cytogenetics10, 273–285 (1971)
Littlefield, L. G., Goh, G. H.: Cytogenetic studies in control men and women. Cytogenet. Cell Genet.12, 17–33 (1973)
Nuzzo, F., Simoni, G., Mottura, A., Larizza, L., Stefanini, M., Dambrosio, F., Zuffardi, O., Maraschio, P.: Deviant karyotypes in amniotic fluid cell cultures: a problem in prenatal diagnosis. J. Genet. Hum.23, 225–232 (1975)
Schmid, W.: Cytogenetical problems in prenatal diagnosis. Hereditas86, 37–44 (1977)
Tenchini, M. L., Giorgi, R., Crimaudo, C., Simoni, G., Nuzzo, F., De Carli, L.: Approaches to examination of genetic damage after a major hazard in chemical industry: preliminary cytogenetic findings on TCDD-exposed subjects after Seveso accident. Presented at The Expert Conference of Genetic Damage by Environmental Factors. In: European Society of Human Genetics. Oslo Symposium, May 14–15, 1977
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Simoni, G., Larizza, L., Sacchi, N. et al. Chromosome lesions in amniotic fluid cell cultures. Hum Genet 49, 327–332 (1979). https://doi.org/10.1007/BF00569352
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00569352