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Current Genetics

, Volume 14, Issue 2, pp 171–176 | Cite as

Comparative transcription analysis of higher plant plastome mutants

  • K. Steiner
  • F. Baldauf
  • M. Metzlaff
  • R. Hagemann
Original Articles

Summary

Several plastome mutants ofPelargonium zonale hort. are characterized by either plastid ribosome deficiencies of specific defects in their photosystems. Southern analyses of plastid DNA fragments containing both the rDNA region and therbcL gene revealed no differences between wild-type and mutant leaves. Northern analyses of mutants with plastid ribosome deficiencies indicate lower levels of plastid ribosomal RNA and mRNA coding for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in mutant leaves relative to green leaves. The data presented in this paper indicate a defect at the transcriptional level in the plastid ribosome deficient plants. We suggest that a RNA polymerase defect affects transcriptional efficiency in these mutants.

Key words

Pelargonium Plastome mutants Plastid RNA Northern analysis 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • K. Steiner
    • 1
  • F. Baldauf
    • 1
  • M. Metzlaff
    • 1
  • R. Hagemann
    • 1
  1. 1.Wissenschaftsbereich Genetik der Martin-Luther-UniversitätSektion BiowissenschaftenHalleGerman Democratic Republic

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