Abstract
The second intron of the rat SVS IV gene contains a tandem repeat region of 20-bp sequences. This region was amplified using the polymerase chain reaction to detect variations. Three alleles, characterized by amplified fragments of 750, 490, and 390 bp, respectively, were found in 24 strains examined. This variation segregated in F1 and backcross progeny in an autosomal codominant manner. We tentatively designated this locusSvs-4. Analysis of linkages between theSvs-4 locus and other loci revealed that it was closely linked to theSvp-1 (<2.9%) and the a (10.0±6.7%) loci, which belong to rat linkage group IV. TheSvp-1 andSvs-4 loci, however, were differently distributed among the inbred rat strains.
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This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
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Kobayashi, E., Ikadai, H., Imamichi, T. et al. A polymorphism detected in a variable number of tandem repeats (VNTR) of the seminal vesicle secretion (SVS) IV gene in inbred rats. Biochem Genet 30, 339–346 (1992). https://doi.org/10.1007/BF00569324
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DOI: https://doi.org/10.1007/BF00569324