Summary
Mammary duct-segments, each 0.6 mm long, from virgin female mice were isografted into the mammary gland-free fat pads of young female mice by Hoshino's quantitative transplantation technique. The hosts were sacrificed at intervals from 24 hous to 21 days. No recognizable mammary structures were recovered earlier than 72 hours after transplantation. In hosts treated with estrogen and progesterone before and after transplantation, regenerating mammary gland tissues were recovered from just over 50% of the grafts, whether or not the donor was pretreated with 3-methylcholanthrene. Mitoses were more often observed in regenerating mammary glandular tissues, which had been transplanted from donors pretreated intraperitoneally with 20 mg of 3-methylcholanthrene nine hours before donation to hormone-treated hosts, and some of the mitoses exhibited chromosomal bridges and polyploid mitoses. Estrogen-progesterone enriched environment apparently enhanced regeneration of mammary transplants. The present experiments suggest that the transplanted mammary tissue seemed to dissociate to individual cells before 72 hours following transplantation and regenerate from them into single mammary glands, and that one dose of 3-methylcholanthrene may cause histologically-visible changes in epithelial cells of rerenerating mammary grafts.
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Biesele, J. J., Grey, C. E., Mottram, F. C.: Some early effects of carcinogenic hydrocarbons on mouse skin. Ann. N. Y. Acad. Sci.63, 1303–1320 (1956).
Bresciani, F.: A comparison of the cell generative cycle in normal, hyperplastic and neoplastic mammary gland in C3H mouse. In: Cellular radiation biology, p. 547–557. Baltimore, Maryland: The William & Wilkins Co. 1965.
Daniel, C. W., DeOme, K. B.: Growth of mouse mammary glands in vivo after monolayer culture. Science149, 634–636 (1965).
——, Young, J. T., Blair, P. B., Faulkin, L. J., Jr.: The in vivo life span of normal and preneoplastic mouse mammary glands: A serial transplantation study. Proc. nat. Acad. Sci. (Wash.)61, 52–60 (1968).
Dao, T. L., Bock, F. G., Crouch, S.: The level of 3-methylcholanthrene in mammary glands of rats after intragastric instillation of carcinogen. Proc. Soc. exp. Biol. (N. Y.)102, 635–638 (1959).
—, Greiner, M. J.: Mammary carcinogenesis by 3-methylcholanthrene. II. Inhibitory effect of pregnancy and lactation on tumor induction. J. nat. Cancer Inst.25, 991–1003 (1960).
—, Sunderland, H.: Mammary carcinogenesis by 3-methylcholanthrene. I. Hormonal aspects in tumor induction and growth. J. nat. Cancer Inst.23, 567–585 (1959).
DeOme, K. B., Faulkin, L. J., Jr., Bern, H. A., Blair, P. B.: Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice. Cancer Res.19, 515–520 (1959).
Faulkin, L. J., Jr., DeOme, K. B.: The effect of estradiol and cortisol on the transplantability and subsequent fate of normal, hyperplastic, and tumorous mammary tissue of C3H mice. Cancer Res.18, 51–56 (1958).
Fischer, A.: The theory of the developmental physiology of malignant tumors. Amer. J. Cancer31, 1–21 (1937).
Hoshino, K.: Morphogenesis and growth potentiality of mammary glands in mice. 1. Transplantability and growth potentiality of mammary tissue of virgin mice. J. nat. Cancer Inst.29, 835–851 (1962).
—: Influence of androgen and estrogen upon morphogenesis of mammary glands in mice. Proc. Canad. Fed. Biol. Soc.6, 30–31 (1963a).
—: Morphogenesis and growth potentiality of mammary glands in mice. II. Quantitative transplantation of mammary glands of normal male mice. J. nat. Cancer Inst.30, 585–591 (1963b).
—: Comparative effects of estrogen, progesterone und prolactin upon mammary growth in mice. Anat. Rec.148, 377–378 (1964a).
—: Regeneration and growth of quantitatively transplanted mammary glands of normal female mice. Anat. Rec.150, 221–236 (1964b).
—: Strain differences in normal and abnormal pregnancies in mice and relationship to mammary tumor incidence. J. nat. Cancer Inst.32, 323–338 (1964c).
—: Transplantability of mammary glands in brown fat pads of mice. Nature (Lond.)213, 194–195 (1967).
—, Bernet, M. A.: 3-methylcholanthrene concentration and clearance in some adipose tissues in mice. Cancer Res.24, 648–651 (1964).
Hoshino, K., Gardner, W. U.: Growth potentialities of the normal mammary tissue in mice. Anat. Rec.139, 240 (1961).
——: Transplantability and life span of mammary gland during serial transplantation in mice. Nature (Lond.)213, 193–194 (1967).
——, Pawlikowski, R. A.: The incidence of cancer in quantitatively transplanted mammary glands and its relation to age and milk agent of the donor and host mice. Cancer Res.25, 1792–1803 (1965).
Hoshino, K., Williams, M. F.: Early histogenesis of transplanted mouse mammary glands: Effect of 3-methylcholanthrene-pretreatment and estrogen-progesterone supplementation. Proc. 48th Meet. Endocrine Soc.127 (1966).
Huggins, C., Grand, L. C., Brillantes, F. P.: Mammary cancer induced by a single feeding of polynuclear hydrocarbons, and its suppression. Nature (Lond.)189, 204–207 (1961).
Kato, R.: Chromosome breakage induced by a carcinogenic hydrocarbon in Chinese hamster cells and human leukocytes in vitro. Hereditas (Lond.)59, 120–141 (1968).
Kirchibaum, A., Williams, W. C., Bittner, J. J.: Induction of mammary cancer with methylcholanthrene. 1. Histogenesis of induced neoplasm. Cancer Res.6, 354–362 (1946).
Mühlbock, O.: The hormonal genesis of mammary cancer. Advan. Cancer Res.4, 371–391 (1956).
Prehn, R. T.: Tumors and hyperplastic nodules in transplanted mammary glands. J. nat. Cancer Inst.13, 859–871 (1953).
Shimkin, M. B., Wyman, R. S., Andervont, H. B.: Mammary tumors in mice following transplantation of mammary tissue. J. nat. Cancer Inst.7, 77–78 (1946).
Symposium on Fundamental Cancer Research: Genetics and Cancer p. 477. Austin, Texas: Univ. Texas Press 1959.
Traurig, H. H., Morgan, C. F.: Autoradiographic investigation of mouse mammary growth by the incorporation of tritiated thymidine into its epithelial components. Anat. Rec.142, 286–287 (1962).
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Supported by grants from the National Cancer Institute of Canada, and the Jane Coffin Childs Memorial Fund for medical Research and also by the Public Health Service fellowship grants to M. F. W. (PHS CRT 5055 and 5Tl CA 5055-06).
Data were used for the Thesis presented to Yale University School of Medicine in partial fulfillment of the requirement for M. D. degree.
Our sincere greatitude to Dr. William U. Gardner, Professor of Anatomy, Yale University School of Medicine, for his interest and valuable suggestions.
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Williams, M.F., Hoshino, K. Early histogenesis of transplanted mouse mammary glands. Z. Anat. Entwickl. Gesch. 132, 305–317 (1970). https://doi.org/10.1007/BF00569268
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DOI: https://doi.org/10.1007/BF00569268