Summary
DNA replication of cultured fibroblasts of early passage derived from Werner's syndrome (adult progeria) patients and from normal subjects were compared by DNA fiber autoradiography. The frequency of replication initiation was decreased in Werner's syndrome cells derived from five patients compared with that in normal cells derived from three persons of different ages. The rate of DNA chain elongation did not differ between Werner's syndrome cells and normal cells.
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References
Björneerg A (1976) Werner's syndrome and malignancy. Acta Dermatovener 56:149–150
Epstein CJ, Martin GM, Schultz AL, Motulsky AG (1966) Werner's syndrome: A review of its symptomatology, natural history, pathologic feature, genetics and relationship to the natural aging process. Medicine 45:177–221
Fujiwara Y, Higashikawa T, Tatsumi M (1977) A retarded rate of DNA replication and normal level of DNA repair in Werner's syndrome fibroblasts in culture. J cell Physiol 92:365–374
Goldstein S (1979) Studies on age-related disease in cultured skin fibroblasts. J Invest Dermatol 73:19–23
Goto M, Horiuchi Y, Tanimoto K, Ishii T, Nakashima H (1978) Werner's syndrome: Analysis of 15 cases with a review of the Japanese literature. J Am Geriat Soc 16:341–347
Goto M, Tanimoto K, Horiuchi Y, Sasazuki T (1981) Family analysis of Werner's syndrome: A survey of 42 Japanese families with a review of the literature. Clin Genet 19:8–15
Hand R (1978) Eucaryotic DNA: Organization of the genome for replication. Cell 15:317–325
Hand R, German J (1975) A retarded rate of DNA chain growth in Bloom's syndrome. Proc Natl Acad Sci USA 72:758–762
Hand R, Tamm I (1974a) DNA replication: Initiation and rate of chain growth in mammalian cells. In: Padilla GM, Cameron IL, Zimmerman A (eds) Cell cycle controls. Academic Press, New York, pp 273–288
Hand R, Tamm I (1974b) Initiation of DNA replication in mammalian cells and its inhibition by reovirus infection. J Mol Biol 82:175–183
Heenen M, Galand P (1980) Decreased rate of DNA-chain growth in human basal cell carcinoma. Nature 285:265–267
Hori T, Lark KG (1973) Effect of puromycin on DNA replication in Chinese hamster cells. J Mol Biol 77:391–404
Huberman JA, Riggs AD (1968) On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol 32:327–341
Lark KG, Consigli R, Toliver A (1971) DNA replication in Chinese hamster cells: Evidence for a single replication fork per replicon. J Mol Biol 58:873–875
Martin GM (1978) Genetic syndromes in man with potential relevance to the pathobiology of aging. Birth Defects 14(1):5–39
Martin GM, Sprague CA, Epstein CJ (1970) Replicative life span of cultivated human cells: Effects of donor's age, tissue and genotype. Lab Invest 23:86–91
Matsumura T, Mitsui Y, Fujiwara Y, Ishii T, Shimada H (1980) Cell, tissue and organ banks in Japan with special reference to the study of premature aging. Japan J Exp Med 50:321–328
Norwood TH, Hoehn H, Salk D, Martin GM (1979) Cellular aging in Werner's syndrome: A unique phenotype? J Invest Dermatol 73: 92–96
Ockey CH (1979) Quantitative replicon analysis of DNA synthesis in cancer-prone conditions and the defects in Bloom's syndrome. J Cell Sci 40:125–144
Pates TD, Farber RA, Tarrant GM, Holliday R (1974) Altered rate of DNA replication in aging human fibroblast cultures. Nature 251: 434–436
Rabbiosi G, Borroni G (1975) Werner's syndrome: Seven cases in one family. Dermatologica 158:355–360
Robert GM, Oppenheim A (1977) Initiation points for DNA replication in nontransformed and simian virus 40-transformed Chinese hamster lung cells. Cell 11:859–869
Salk D, Bryant E, Au K, Hoehn H, Martin GM (1981) Systemic growth studies, cocultivation, and hybridization studies of Werner syndrome cultured skin fibroblasts. Hum Genet 58:310–316
Sheinin R, Hubert J, Pearlman RE (1978) Some aspects of eukaryotic DNA replication. Ann Rev Biochem 47:277–316
Tanaka K, Nakazawa T, Okada Y, Kumahara Y (1979) Increase in DNA synthesis in Werner's syndrome cells by hybridization with normal human diploid and HeLa cells. Exp Cell Res 123:261–267
Welch BL (1949) Further note on Mrs Aspin's Tables and on certain approximations to the tabled function. Biometrika 36:293–296
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Takeuchi, F., Hanaoka, F., Goto, M. et al. Altered frequency of initiation sites of DNA replication in Werner's syndrome cells. Hum Genet 60, 365–368 (1982). https://doi.org/10.1007/BF00569220
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DOI: https://doi.org/10.1007/BF00569220