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A simple reproducible method for prometaphase chromosome analysis

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Summary

A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase.

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Author notes

  1. Joseph Rybak

    Present address: Department of Pathology, UTHSC-Dallas, 75235, Dallas, Texas

  2. Avirachan Tharapel

    Present address: Department of Pediatric, Children's Hospital of Buffalo, SUNYAB, 219 Bryant Street, 14222, Buffalo, New York

Authors and Affiliations

  1. Genetics Screening and Counseling Service, Texas Department of Mental Health and Mental Retardation, 76201, Denton, Texas, USA

    Joseph Rybak, Avirachan Tharapel, Sheldon Robinett, Mary Garcia, Carl Mankinen & Mahlon Freeman

Authors
  1. Joseph Rybak
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  2. Avirachan Tharapel
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  3. Sheldon Robinett
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  4. Mary Garcia
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  5. Carl Mankinen
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  6. Mahlon Freeman
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Rybak, J., Tharapel, A., Robinett, S. et al. A simple reproducible method for prometaphase chromosome analysis. Hum Genet 60, 328–333 (1982). https://doi.org/10.1007/BF00569213

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  • DOI: https://doi.org/10.1007/BF00569213

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