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A comparative study of Nickel hypersensitivity by the lymphocyte transformation test in atopic and non-Atopic dermatitis

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Summary

In spite of the non-specific mitogenic effect of nickel salts,3H-thymidine uptake ratio (TUR) of nickel-stimulated lymphocyte cultures in nickel-sensitive atopic dermatitis patients and in non-atopic nickel-sensitive patients was significantly higher at 10 µg per ml concentration of nickel salts than in nickel non sensitive subjects (P < 0.001). A significant difference was also demonstrated at concentrations of nickel salts of 40 µg per ml.

The TUR value of nickel-stimulated cultures in nickel-sensitive atopic dermatitis patients tended to decrease in comparison to non-atopic nickel-sensitive patients. Furthermore, the TUR of phytohemagglutinin-p (PHA) stimulated cultures was also lower in atopic dermatitis patients than in non-atopic subjects.

Zusammenfassung

Trotz der unspezifischen Mitogenität von Nickelsalzen in der Lymphocyten-Kultur, war die3H-Thymidin-Einbaurate (TUR) nickelstimulierter Lymphocyten-Kulturen bei Nickelallergikern mit und ohne atopischer Dermatitis bei einer Nickelsalzkonzentration von 10 µg bzw. 40 µg/ml signifikant höher als bei Kontrollpersonen ohne Nickelallergie (P < 0.001).

Die TUR in nickelstimulierten Kulturen von nickelallergischen Atopikern war etwas geringer als bei Nicht-Atopikern mit Nickelallergie. Weiterhin war die TUR in Phytohämagglutinin-stimulierten Kulturen niedriger bei Patienten mit atopischer Dermatitis als bei Nicht-Atopikern.

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References

  1. Aspegren, N., Rorsman, H.: Short-term culture of leucocytes in nickel hypersensitivity. Acta dermat.-venereol. (Stockh.)42, 412–417 (1962)

    Google Scholar 

  2. Burger, D. R., Lilley, D. P., Vetto, R. M.: Lymphocyte suppression associated with alpha globulin changes in cellular immunity. Cell. Immunol.10, 432–441 (1974)

    Google Scholar 

  3. Epstein, S.: Contact dermatitis due to nickel and chromate: observations on dermal delayed (tuberculin-type) sensitivity. Arch. Derm.73, 236–255 (1956)

    Google Scholar 

  4. Fjelde, A., Kopecka, B.: Cell transformation and mitogenic effects in blood leucocyte cultures of atopic dermatitis patients. Acta dermat.-venereol. (Stockh.)47, 168–176 (1967)

    Google Scholar 

  5. Forman, L., Alexander, S.: Nickel antibodies. Brit. J. Derm.87, 320–326 (1972)

    Google Scholar 

  6. Gimenez-Camarasa, J. M., Garcia-Calderon, P., Asensio, J., De Moragas, J. M.: Lymphocyte transformation test in allergic contact nickel dermatitis. Brit. J. Derm.92, 9–15 (1975)

    Google Scholar 

  7. Hinrichs, D. J., Gibbins, B. L.: The in vitro detection of antigen sensitivity in contact dermatitis by lymphocyte transformation. Cell. Immunol.18, 343–350 (1975)

    Google Scholar 

  8. Hutchinson, F., Raffle, E. J., Macleod, T. M.: The specificity of lymphocyte transformation in vitro by nickel salts in nickel sensitive subjects. J. invest. Derm.58, 362–365 (1972)

    Google Scholar 

  9. Jones, H. E., Lewis, C. W., McMarlin, S. L.: Allergic contact sensitivity in atopic dermatitis. Arch. Derm.107, 217–222 (1973)

    Google Scholar 

  10. Lobitz Jr., W. C., Honeyman, J. F., Winkler, N. W.: Suppressed cell-mediated immunity in two adults with atopic dermatitis. Brit. J. Derm.86, 317–328 (1972)

    Google Scholar 

  11. Malten, K. E.: Nickel-allergic contact dermatitis and atopy. Dermatologica (Basel)142, 113–116 (1971)

    Google Scholar 

  12. Millikan, L. E., Conway, F., Foote, J. E.: In vitro studies of contact hypersensitivity: lymphocyte transformation in nickel sensitivity. J. invest. Derm.60, 88–90 (1973)

    Google Scholar 

  13. Mirza, A. M., Perera, M. G., Maccia, C. A., Dziubynskyj, O. G., Bernstein, I. L.: Leucocyte migration inhibition in nickel dermatitis. Int. Arch. Allergy49, 782–788 (1975)

    Google Scholar 

  14. Morgan, J. K.: Observations on the persistence of skin sensitivity with reference to nickel eczema. Brit. J. Derm.65, 84–94 (1953)

    Google Scholar 

  15. Pappas, A., Orfanos, C. E., Bertram, R.: Non-specific lymphocyte transformation in vitro by nickel acetate: a possible source of errors in lymphocyte transformation test (LTT). J. invest. Derm.55, 198–200 (1970)

    Google Scholar 

  16. Pentycross, C. R.: Technique for lymphocyte transformation. J. Clin. Path.21, 175–178 (1968)

    Google Scholar 

  17. Rajka, G.: Delayed dermal and epicutaneous reactivity in atopic dermatitis (prurigo Besnier): I. Delayed reactivity to bacterial and mold allergens. Acta dermat.-venereol. (Stockh.)47, 158–162 (1967)

    Google Scholar 

  18. Sulzberger, M. B.: Allergic contact sensitivity in atopic dermatitis. In: Editorial remarks: Year book of dermatology, pp. 97–98. Chicago: Year Book Publisher Inc. 1974

    Google Scholar 

  19. Strassburger, D.: Lymphocyten-Transformationstest mit Nickelacetat: Ist Nickelacetat ein unspezifisches Mitogen? Vortrag auf der III. Jahrestagung der Arbeitsgemeinschaft Dermatologische Forschung, Berlin 1975

  20. Schöpf, E., Böhringer, D.: IgE and cell-mediated immunity in atopic dermatitis. J. Derm. (Japan)1, 133–144 (1974)

    Google Scholar 

  21. Schöpf, E.: Störungzellvermittelter Immunreaktionen bei Neurodermatitis atopica: verminderte Spontanrosettenbildung von T-Lymphozyten. Dermatologica (Basel)149, 210–219 (1974)

    Google Scholar 

  22. Uehara, M., Takahashi, C., Ofuji, S.: Pustular patch test reactions in atopic dermatitis. Arch. Derm.111, 1154–1157 (1975)

    Google Scholar 

  23. Wells, G. C.: Effects of nickel on the skin. Brit. J. Derm.68, 237–242 (1956)

    Google Scholar 

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Authors and Affiliations

  1. Abt. Dermatologie II, Schwerpunkt: Allergien, Klinikum der Universität Heidelberg, Hautklinik, Heidelberg 1, Germany

    C. W. Kim

  2. Berufskrankheiten der Haut, Voßstraße 2, D-6900, Heidelberg 1, Germany

    E. Schöpf

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  1. C. W. Kim
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  2. E. Schöpf
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Kim, C.W., Schöpf, E. A comparative study of Nickel hypersensitivity by the lymphocyte transformation test in atopic and non-Atopic dermatitis. Arch. Derm. Res. 257, 57–65 (1976). https://doi.org/10.1007/BF00569114

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  • DOI: https://doi.org/10.1007/BF00569114

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