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Stimulation by melanocyte stimulating hormone and dibutyryl adenosine 3′,5′-cyclic monophosphate of DNA synthesis in human melanocytes in vitro

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Summary

The proliferation of human melanocytes in vitro was stimulated by MSH. This stimulation was further intensified by the simultaneous addition of theophylline with MSH. Theophylline alone stimulated proliferation moderately. Dibutyryl cyclic AMP strongly stimulated the proliferation, but sodium butyrate, 5′-AMP and cGMP did not. The stimulation by dibutyryl cyclic AMP was continued up to 4 days so far tested.

These findings are directly opposed to those on mouse melanoma cells in culture which responded with retarded growth to MSH and cyclic AMP. It is suggested that the difference of proliferation control may explain the different reaction of the melanocyte and the melanoma cells. The epidermal melanocytes seem to belong to the exceptional group of the cells which responded to cyclic AMP with accelerated prohferation.

Zusammenfassung

Die Proliferation der menschlichen Melanocyten in der Kultur wird stimuliert von MSH. Eine weitere Stimulation wird durch die gleichzeitige Zugabe von Theophyllin zum MSH erreicht. Theophyllin alleine bewirkt eine mäßige Proliferationssteigerung. Dibutyryl zyklisches AMP ist ein stärkerer Stimulator im Gegensatz zum Natriumbutyrat, 5′-AMP und cGMP. Der Effekt von Dibutyryl zyklisches AMP war über 4 Tage hin nachweisbar. Diese Befunde stehen im Gegensatz zu Ergebnissen der Mausmelanomzellen, welche in der Kultur auf MSH und zyklisches AMP eine Einschränkung der Proliferation erfahren. Als Ursache wird eine unterschiedliche Proliferationskontrolle angeschen. Die epidermalen Melanocyten scheinen zu der besonderen Zellgruppe zu gehören, welche auf zyklisches AMP mit einer beschleunigten Proliferation reagieren.

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Authors and Affiliations

  1. Department of Dermatology Osaka University School of Medicine, Fukushima-ku, Osaka 553, Japan

    Yukio Kitano

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  1. Yukio Kitano
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Supported by research grants from the Ministry of Education, Japan

The author wishes to thank Dr. P. A. Desaulles and Dr. W. Rittel for their provision of α-MSH

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Yukio, K. Stimulation by melanocyte stimulating hormone and dibutyryl adenosine 3′,5′-cyclic monophosphate of DNA synthesis in human melanocytes in vitro. Arch. Derm. Res. 257, 47–52 (1976). https://doi.org/10.1007/BF00569112

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  • DOI: https://doi.org/10.1007/BF00569112

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